[Objective] The research aimed to study the effect of malachite green (MG) on mitosis of Vicia faba root tip cell. [Method] Dif ferent concentrations of malachite green were used to treat Viciafaba root lip cell for 12.24,36,48 and 72 h.The mitosis of Vieia fa a root tip ceil was observed by conventional chromosome compression technology ,and the mitotic index ( MI) of each treatment group was counted. [ Re sult] As the treated time prolonged,MI presented the M-shaped variation trend,which illustrated that Vicia faba root tip had a adaptive process to MC. As the concentration of MC increased,Ml gradually rote. When the concentration of MG was during 0.50 - 1. 00 mg/L, Ml was the maximum. When the concentration of MG further increased,MI decreased. It showed that MG was favorable for the plant growth in the certain concentration range. [Conclusion] The research could provide the basis for the reasonable utilization of MG,and the reference for evaluating the environmental harm of MG.%[目的]研究孔雀石绿对蚕豆根尖细胞有丝分裂的影响.[方法]采用不同浓度的孔雀石绿溶液处理蚕豆根尖细胞12、24、36、48和72 h,通过常规染色体压片技术,观察蚕豆根尖细胞有丝分裂,并计算各处理组的有丝分裂指数.[结果]随着处理时间的延长,有丝分裂指数呈M型变化趋势,说明蚕豆根尖对孔雀石绿有一个适应过程.随着孔雀石绿浓度的升高,有丝分裂指数逐渐升高,在浓度为0.50～1.00 mg/L时,有丝分裂指数整体达到顶点；随着孔雀石绿浓度的进一步升高,有丝分裂指数有所下降,说明孔雀石绿在一定浓度范围内对植物生长有利.[结论]该研究可以为孔雀石绿的合理利用提供依据,并为评价孔雀石绿对环境的危害提供参考.
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