[Objective] To induce the callus from P. Cuspidatum leaves and to determine the content of resveratrol. [ Method]P. Cuspidatum leaves as tissue culture materials were induced on MS induction medium containing 0.2 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 1 mg/ 6-amino-benzyl adenine (6-BA) at 28 ℃ and dark. 15 d later,the induced callus was transferred to the same culture medium for 15 d,which were transferred 3 times. Subsequently,the callus was cut and then cultured in the same medium for three times. The content of resveratrol in the callus induced fromP. Cuspidatum leaves was determined by HPLC. [ Result] The callus induced from P. Cuspidatum leaves grew well,and its resveratrol content was determined at 0.24 mg/g. [Conclusion] The HPLC method was simple. Convenient,quick and accurate to determine the content of resveratrol in P. Cuspidatum leaves.%[目的]诱导虎杖叶的愈伤组织并测定其中白藜芦醇的含量.[方法]以虎杖叶为组织培养材料,在28℃和黑暗培养条件下,在含有浓度0.2 mg/L2,4-二氯苯氧乙酸(2,4-D)和浓度1 mg/L6-苄氨基腺嘌呤(6-BA)的MS诱导培养基中进行诱导,15 d后把诱导出的愈伤组织转接到相同培养基中培养15d,转接3次后,把愈伤组织切下放置在相同培养基上继续培养3次后,采用HPLC法测定虎杖叶愈伤组织中白藜芦醇的含量.[结果]诱导出的愈伤组织生长状况良好,并且测得虎杖叶愈伤组织中白藜芦醇的含量为0.24 mg/g.[结论]由叶诱导出的愈伤组织中含有白藜芦醇成分,用HPLC法测定其含量,方法简便、迅捷,结果可靠.
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