[Objective] The aim was to determine the contents of p-elemene in different parts of Eupatorium adenophorum Spreng. [ Method] Contents of p-elemene in different parts of E. Adenophorum grown in Panxi Area of China were measured by HPLC. The chromatographic conditions were as follows;Eclipse XDB-C18(4.6 mm × 150 nun,5μm) ,1.0 ml/min flow rate,210 nm detective wavelength,ethanol-acetonitrile-water as (70:10=20, V/V/V)mobile phase,20 μl injection volume,30 ℃column temperature. [Result] p-elemene existed in all parts of E. Adenophorum plant. And the content was the maximum in leaves. In the naturally dry leaves,β-elemene content was 0. 083% , which was more than 3 times higher than the content in Curcuma wenyujin Y. H. Chen. [ Conclusion] Extracting p-elemene from E. Adenophorum could greatly reduce the material cost of p-elemene, and could develop new methods for the control of malignant weeds in China.%[目的]测定紫茎泽兰不同生长部位中抗肿瘤活性成分β-榄香烯的含量.[方法]采用HPLC法对攀西地区产的紫茎泽兰(Eupatorium adenophorum Spreng)不同生长部位中β-榄香烯的含量进行测定.色谱条件:色谱柱为Eclipse XDB-C18(4.6mm×150 mm,5μm),流动相为乙醇-乙腈-水(70∶10∶20V/V/V),流速为1.0 ml/min,检测波长为210nm,进样量为20μl,柱温为30℃.[结果]紫茎泽兰植株各部位均含有活性成分β-榄香烯,且叶片中的含量最高.自然晒干叶中β-榄香烯含量为0.083％,比中药温莪术中的含量高3倍以上.[结论]从紫茎泽兰中提取β-榄香烯,不仅可大幅度降低β-榄香烯的原料成本,还可为我国恶性杂草植物的治理和开发利用开辟新的途径.
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