[目的]测定构树叶中牡荆素和芹菜素-7-O-β-D-葡萄糖醛酸苷的含量.[方法]采用HPLC色谱法,以无水甲醇-浓度1％乙酸(30:70,V/V)为流动相,359 nm为检测波长,测定构树叶中牡荆素的含量；以无水甲醇-浓度0.05％磷酸(40:60,V/V)为流动相,324 nm为检测波长,测定芹菜素-7-O-β-D-吡喃葡萄糖醛酸苷的含量.[结果]牡荆素在0.056～0.488 μg/ml范围内线性关系较好,相关系数R=0.9992:平均回收率为99.2％,RSD为1.42％；芹菜素-7-O-β-D-吡喃葡萄糖醛酸苷在1.808～4.068μg/ml范围内线性关系良好,相关系数R=0.999 8.平均回收率为100.9％,RSD为1.58％.[结论]该方法简便、分离效果好,可用于构树叶质量标准的制订.%[Objective]To determine the content of vitexin and apigenin-7-O-β-D-pyranglycuronide in Broussonetia papyrifera leaves. [ Method] Using absolute methanol-1% acetic acid glacial (30:70,V/V) as mobile phase,the content of vitexin in B. Papyrifera leaves was measured by HPLC method at the detection wavelength of 359 nm. Using absolute methanol-0.05% phosphoric acid(40:60,V/V) as mobile phase,the content of apigenin-7- O-β-D-pyranglycuronide was measured by HPLC method at the detection wavelength of 324 nm. [Result] There was a good linearity in the concentration range of 0.056 - 0.488 μg/ml for vitexin ( R = 0. 999 2). The average recovery of vitexin was 99. 2% with the RSD of 1.42%. Apigenin-7-O-μ-D-pyranglycuronide presented a good linearity in the concentration range of 1. 808 - 4. 068 μg/ml (R = 0.999 8 ). The average recovery of apigenin-7-O-μ-D-pyranglycuronide was 100.9% with the RSD of 1.58%. [ Conclusion ] This method that takes advantages of simplicity and good separating effect could be used for establishing the quality standard of B. Papyrifera leaves.
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