[Objective]The study aimed to compare the effects of different pretreatment methods on the extraction of lycopene and optimize its pretreatment technology. [Method]The tomato pulp samples was pretreated by the methods such as centrifugal dewatering, freezing, drying and alcohol treatment for studying the effects of pretreatment methods on the extraction of lycopene. [Result]The extracts had absorption peaks at 444, 472 and 502 nm resp. and its maximum absorption was at 472 nm. The extraction rates of lycopene from all the pretreated tomato pulps were as follows: centrifugal dewatering treatment ＞ freezing treatment ＞ stoving at 45 ℃ and grinding ＞ stoving at 60 ℃ and grinding and the extraction rate of lycopene from tomato pulp pretreated with anhydrous alcohol was a little lower than that of lycopene from non-pretreated tomato pulp. The tomato pulp was treated by centrifugal dewatering and freezing and then extracted with petroleum ether ∶ acetone=1∶1 for 1 h, the absorbance of extracts changed less. The tomato pulp was treated by drying and then extracted with organic solvent for 1 h, the absorbance of extracts changed more. When the material-liquid ratio was 1∶6, the extraction effect on lycopene was better. [Conclusion]After the tomato fruit was pulp beaten, both centrifugal dewatering and freezing treatments could increase extraction rate and shorten extraction time.%[目的]比较不同预处理方法对番茄红素提取的影响,优化其预处理工艺.[方法]采用离心脱水、冷冻、干燥、乙醇处理等方法对番茄浆样进行预处理,研究预处理方法对番茄红素提取的影响.[结果]提取液在444、472和502 nm处各有1个吸收峰,其最大吸收在472 nm.各预处理番茄浆的番茄红素提取率高低为:离心脱水处理＞冷冻处理＞ 45 ℃烘干、粉碎＞ 60 ℃烘干、粉碎,无水乙醇处理稍低于不处理番茄浆.番茄浆经离心脱水和冷冻处理后用石油醚∶丙酮=1∶1提取1 h,提取液的吸光度变化较小,番茄浆经干燥处理再用有机溶剂提取1 h,提取液的吸光度变化较大.料液比为1∶6时,番茄红素的提取效果较好.[结论]番茄果实打浆后,离心脱水处理或冷冻处理均可提高提取率,缩短提取时间.
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