[Objective] HPLC of VC in kiwifru was established. [Method] An Supelco C8 column(25 cm×4.6 mm,5 μm) was used for separation,with methanol-0.5% phosphoric acid (8∶75) as mobile phase and detected at 258 nm.[Result] The linear range was 0.324-5.120 μg (r=0.998),and average recovery was 99.87%, and RSD was 1.8%. [Conclusion] The results of determination were stable and simple.It Can be used to simutaneously determine vitamin C in kiwifru.%[目的]建立猕猴桃中VC的高效液相色谱测定法.[方法]以甲醇-0.5%磷酸(8∶75)为流动相,用Supelco C8柱(25 cm×4.6 mm,5 μm)进行分离,于波长258 nm处检测.[结果]线性范围为0.324~5.120 μg (r=0.998),回收率为99.87%,RSD为1.8%.[结论]用该方法测定VC含量,结果稳定,重现性好,可操作性强,适于猕猴桃中Vc含量的质量控制.
展开▼
