[Objective] To provide a theoretical basis and reference for the clinical use of the effective components of Astragalus,the present study detected the Astragalus Polysaccharide and Astragaloside effect on IFN-γ secretion of RIMVECs.[Methods] Astragalus saponins and Astragalus polysaccharide are used to induce the IFN-γ secretion of RIMVECs.The IFN-γ are detected with ELISA method.[Results] The expression IFN-γ in RIMVECs gradually increased over time in 25 ug/mL and 50 ug/mL Astragaloside treated group,it reached the peak after 24 h with obvious enrichment effect;the expression of IFN-γ was also gradually increased over time in 25 ug/mL Astragalus polysaccharide treated group,it reached the highest expression level after 24 h,50 ug/mL Astragalus polysaccharide could also increase the IFN-γ expression over time with a peak after 12 h,then the expression level decreased.[Conclusion] The results suggested that the immunomodulatory effects of Astragaloside and Astragalus polysaccharide might induce the IFN-γ secretion of RIMVECs,which provided experimental basis for the clinical application of Astragalus membranaceus.%[目的]探明黄芪甲苷和黄芪多糖能否诱导RIMVECs分泌IFN-γ,为黄芪及有效成分的临床应用提供理论和参考依据.[方法]以体外培养的RIMVECs为模型,用ELISA法检测不同浓度的黄芪甲苷和黄芪多糖在不同时间诱导RIMVECs分泌IFN-γ的表达量.[结果]25 μg/mL和50 μg/mL黄芪甲苷能诱导RIMVECs表达IFN-γ,且随着时间延长表达逐渐升高,24 h时达到峰值,具有明显的富集效应;25 μg/mL和50 μg/mL黄芪多糖也能诱导RIMVECs表达IFN-γ,随着时间延长RIMVECs细胞上清液中IFN-γ表达量逐渐增多,25 μg/mL组IFN-γ表达量24 h达到峰值;50 μg/mL组IFN-γ表达量12h达到峰值,随后表达量降低.[结论]结果提示,黄芪甲苷和黄芪多糖的免疫调节作用可能是通过诱导RIMVECs分泌IFN-γ来实现,为黄芪的临床应用提供了试验依据.
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