To investigate the effect of regenerated silk fibroin on the differentiation and maturation of murine BM-derived DCs in vitro.In order to clarify the immunogenicity and biocompatibility of silk fibroin.Murine BM-derived DCs were Isolated and cultured in vitro and then transferred to the wells coated with silk fibroin.The cell morphology was observed with microscope.And the proliferation of the cells from each group was determined by MTT assay.The expressions of associated molecules were analyzed with flow cytometry. Results showed that (1)Both regenerated Antheraea pernyi silk fibroin and regenerated Bombyx mori silk fibroin could support the grow and aggregate formation of murine DCs;(2)MTT assay showed that regenerated Antheraea pernyi silk fibroin could support the proliferation of DC2.4 significantly;(3)By FCM analyses,the expression pattern of CD11c,CD40,CD80,CD86 and MHC -Ⅱmolecules on BM-derived DCs were similar between silk fibron group and blank group.Regenerated Antheraea pernyi silk fibroin can support the growth and proliferation of DC2.4 better than regenerated Bombyx mori silk fibroin did.Regenerated silk fibroin can not promote the maturation of murine BM-derived DCs.%体外研究再生丝素蛋白材料对小鼠骨髓来源树突状细胞分化、成熟的影响，探讨丝素蛋白的免疫原性及作为生物材料的意义。体外分离培养小鼠骨髓来源树突状细胞，接种于铺有再生丝素蛋白生物材料的细胞培养板中培养，在光学显微镜下观察细胞形态；FCM分析细胞表面相关分子的表达。同时用 MTT 法测小鼠骨髓来源树突状细胞细胞株 DC2．4在所选各种材料表面的生长增殖情况。结果表明：（1）再生柞蚕丝素蛋白和家蚕丝素蛋白均可支持小鼠骨髓来源的 DCs 生长和聚集；（2）MTT 结果表明再生柞蚕丝素蛋白与再生家蚕丝素蛋白相比可促进小鼠 BM来源 DC2．4的增殖；（3）再生柞蚕丝素蛋白和再生家蚕丝素蛋白上的小鼠 BM来源 DCs 表面低表达 CD11c、CD40、CD80、CD86、MHC -Ⅱ分子，与空白对照组 DCs 的表达谱相比无明显差别。再生柞蚕丝素蛋白与再生家蚕丝素蛋白相比，可较好地支持小鼠 BM来源 DC2．4的生长和增殖；再生丝素蛋白无刺激小鼠 BM来源 DCs 成熟的生物学效应。
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