Objective We aimed to identify key genes and pathways of airway epithelial cells involved in bronchial asthma by comparing genetic information in the databases for patients with bronchial asthma and normal people. Methods To find differentially expressed genes (DEGs), mRNA microarray dataset, GSE43696, of airway epithelial cells in asthma was analyzed by GE02R. Functional and pathway enrichment analyses were performed for DEGs using the DAVID database. The protein-protein interaction networks were established using STRING to identify key genes and important complexes. Results A total of 355 DEGs were identified; of which, 130 were up-regulated and 225, down-regulated. The genes identified were involved in cell movement, growth factor binding, and ion channel activity. Nine key genes were recognized, including BDNF, ERBB2 IL6, VEGFA, KIT, ADCY4, PRKAR2B, CCR6, and NMU. Conclusion All nine key genes identified play important roles in asthma and serve as potential targets for treatment of bronchial asthma.%目的 通过比较支气管哮喘患者气道上皮细胞与正常气道上皮细胞的基因芯片数据,鉴定哮喘气道上皮细胞中的关键基因.方法对哮喘气道上皮细胞基因芯片数据GSE43696进行GE02R分析,筛选出差异表达基因.然后使用DAVID数据库对差异表达基因进行功能及通路富集化分析.利用STRING对差异表达基因进行蛋白相互作用网络图(PPI)分析,并选出关键基因及显著模块.结果共获得个差异表达基因,130个上调基因,225个下调基因,主要涉及细胞运动、生长因子结合离子转运和通道活性.共筛出9个关键基因,包括BDNF,ERBB2,IL6,VEGFA,KIT, ADCY4,PRKR2B,CCR6和NMU.结论筛选的关键基因均在支气管哮喘中起重要作用,为支气管哮喘的诊治提供潜在的治疗目标.
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