The protein extraction solution from the supercritical carbon dioxide defatted corylus mandshurica kernel meal was hydrolyzed by Alcalase,and then the hydrolysate was separated and purified by Sephadex G-25.After that two kernel polypeptide fractions Ⅰ and Ⅱ were prepared,the amino acid composition of thetwo polypeptide fractions was determined,and its nutrition and total reducing power were evaluated.The results showed that,for polypeptide fractions Ⅰ and Ⅱ,the score of ratio coefficient of amino acid was 36.15 and 41.11 each,which showed that the nutritional value wasrelatively low;the limiting amino acidswere Lysine and Leucine for two fractions,respectively.The antioxidant experiment results show that under the same concentration,the total reducing power of polypeptide fraction Ⅱ was higher than that of polypeptide fraction I,but both were less than that of VC.%以超临界CO2脱脂制备出的毛榛榛仁粕为原料,经Alcalase碱性蛋白酶水解榛仁粕蛋白提取液后,采用葡聚糖凝胶G-25对水解液进行分离纯化,得到Ⅰ和Ⅱ2种榛仁多肽组分,测定其氨基酸组成,并对其营养和总还原能力进行了评价.研究结果表明:对于毛榛榛仁多肽组分Ⅰ和Ⅱ,其氨基酸比值系数分值分别为36.15和41.11,营养价值较低,限制氨基酸分别是赖氨酸和亮氨酸.抗氧化实验结果表明,在相同质量浓度条件下,多肽组分Ⅱ的总还原能力要高于多肽组分Ⅰ,但均小于VC.
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