在微波条件下,以4-羟基香豆素、甲醛、环己二酮、硝酸铈胺为原料一锅法高产率的合成系列喹啉-苯并吡喃类衍生物.化合物的结构通过X-ray单晶衍射确定.通过经典的Ellman法,分别测试了20 μM、50 μM浓度条件下所合成化合物对乙酰胆碱酯酶的抑制活性,并系统总结了该类化合物详细的构效关系.其中化合物4ag呈现出很好的抑制活性,IC50值达到5.63 μM.此外,通过对化合物与乙酰胆碱酯酶的分子模拟研究,初步探究了该类化合物可能的作用机理.%An efficient synthesis of chromeno[4,3-b]quinoline derivatives via one-pot,four-component reaction of 4-hydroxycoumarin,formaldehyde,cyclohexanedione,ammonium ceric nitrate under microwave irradiation was accomplished.The structures of these compounds were unambiguously confirmed by single crystal X-ray diffraction.Furthermore,the anti-AChE activities of these compounds in vitro were investigated at concentrations of 20 μM and 50 μM by using a standard Ellman's method.The relationship of inhibitory activities and structures of these chromeno [4,3-b]quinolines was also systematically studied.Of all the compounds investigated,4ag emerged as the most potent AChE inhibitor with IC50 values of 5.63 μM,and it might be used as potent lead for the development anti-AChE agents.Moreover,molecular modelling was conducted to understand the optimal interaction of AChE with these types of compounds.
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