Objective To study the protective effect of propofol on lipopolysaccharide (LPS)-induced Ana-1 cell damage.Methods The LPS-induced inflammation damage of Ana-1 cells was es-tablished as a model.Ana-1 cells were divided into control group(group C),LPS group(group L), LPS+propofol 10μmol/L group(group LP1),LPS+propofol 20μmol/L group(group LP2),LPS+propofol 40 μmol/L group(group LP3),and LPS+propofol 80 μmol/L group(group LP4),respec-tively.Cell survival rate was assessed using MTT,interleukin 1βand 6,and tumor necrosis factors (TNF-α)were determined.Results Survival rate of Ana-1 cells in group L was lower than that in group C,while it was significantly improved in group LP1,LP2,LP3,LP4 in a dose-dependent man-ner (P <0.01);IL-1β,IL-6,TNF-αrelease was significantly higher in group L than in group C;IL-1β,IL-6,TNF-αrelease significantly reduced in group LP1,LP2,LP3,LP4 (P <0.01)in a dose-dependent manner (P <0.01).Within the selected dose range in this experiment,group LP4 had the best protection effect.Conclusion Propofol can reduce inflammation damage of Ana-1 cells caused by LPS.%目的：研究丙泊酚对脂多糖（LPS）引起的小鼠巨噬细胞 Ana-1炎症损伤的影响。方法用脂多糖处理 Ana-1细胞作为细胞损伤模型，将培养的 Ana-1细胞分成六组：对照组（C 组）、LPS 处理组（L 组）、LPS＋丙泊酚10μmol／L 处理组（LP1组）、LPS＋丙泊酚20μmol／L 处理组（LP2组）、LPS＋丙泊酚40μmol／L 处理组（LP3组）、LPS＋丙泊酚80μmol／L 处理组（LP4组）。采用MTT 法测定细胞的存活率，并测定丙泊酚作用前后 IL-1β、IL-6、TNF-α含量。结果 L 组 Ana-1细胞的存活率明显低于 C 组，而 LP1、LP2、LP3、LP4组可明显提高 Ana-1细胞的存活率，且对细胞存活率的提高程度呈剂量递增的效应关系（P ＜0.01）；L 组 IL-1β、IL-6、TNF-α的含量明显高于 C组，而 LP1、LP2、LP3、LP4组可明显降低损伤细胞 IL-1β、IL-6、TNF-α的含量（P ＜0.01），且其降低的程度呈剂量效应关系（P ＜0.01）。在本实验所选择剂量范围内，LP4组的保护效果最佳。结论丙泊酚通过降低炎症损伤来减轻 LPS 诱导的 Ana-1细胞损伤。
展开▼
