Objective To study the pathological lesions of rat liver induced by the Gynura root water decoction, and establish a rat model for hepatic veno- occlusive disease (HVOD). Methods 2100 gram Gynura root was prepared into the water decoction with the concentration of 1.5 g/mi. Group A, B and C with 20 SD rats were perfused intragastrically with the Gynura root water decoction with different concentrations of 15 g · kg-1 · d-1 , 7.5 g · kg-1 · d-1 and 3.75 g· kg-1·d-1 , respectively. Group D was perfused intragastrically with water which served as the controls. The ratio of male to female was 1:1 in every group. The body weights and symptoms were observed every day. Half of the rats from each group were killed and their liver samples were stained with H&E and Masson techniques after one week and two weeks of drug administration respectively. Results The pathological manifestations of HVOD were observed in the 15 rats of 18 (83. 3％ ) in group A, 15 of 20 (75.0％) in group B and 5 of 20 (40.0％) in group C. With the same dose, the female rats were prone to HVOD. After re - grouping by gender, pathological manifestations of HVOD could be found in 23 of the 29 male rats and 12 of the 29 female rats (79.3％ vs 41.3％, P =0.003 ). Conclusion The rat HVOD model could be induced by the Gynura root water decoction successfully which was associated with the concentration and gender.%目的 通过观察不同浓度土三七水煎剂所致的大鼠肝脏病理学变化,建立一种模拟临床患者服药所致肝小静脉闭塞病(HVOD)的动物模型.方法 土三七2100 g水煎制成浓度为1.5 g/ml土三七生药煎剂,SD大鼠76只随机分为A、B、C、D四组,A、B、C组每组20只,分别以土三七水煎剂高(15g·kg-1·d-1)、中(7.5 g·kg-1·d-1)、低(3.75 g·kg-1·d-1)浓度灌胃,D组16只以温开水灌胃作为对照,各组大鼠雌、雄各半.每日测量大鼠体重并记录异常表现,灌胃1周和2周后各组大鼠分别处死各半,肝脏标本行病理检查,HE及Masson染色,光镜下参照Deleve评分标准判定病变及严重程度.结果 土三七高、中、低浓度组分别有83.3%(15/18)、75.0%(15/20)、40.0%(5/20)出现了肝小静脉闭塞病病理表现;相同剂量下雌性较雄性更易发病,雌、雄鼠造模成功率分别为79.3%(23/29)和41.3%(12/29)(P=0.003).结论 土三七水煎剂灌胃可以成功建立HVOD大鼠模型,剂量和大鼠性别与造模成功率直接相关.
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