Objective To investigate the possible impact of the polymorphism in 5 A/6 A Matrix metalloproteinase-3 ( MMP-3) gene promoter on the risk of left ventricle hypertrophy. Methods 104 hypertensives and 36 control subjects were recruited. Polymerase chain reaction followed by restrictionfrag-ment length polymorphism(PCR-RFLP) was used to detect SNP-1171(5A/6A)in MMP-3 gene promoter region. LV mass(LVM) was calculated using theformula: 0. 8× ( 1. 04 × [ ( LVIDd + IVSd + PWd)3 -(LVIDd)3) ] + 0.6. Left ventricular mass index(LVMI) was determined using formula:LVM/BSA, where BSA was the body surface area. Results The distribution of MMP-3 genotype was not significantly different between hypertensive patients and controls. LVMI were significantly higher in the subjects than controls. Genotype 6A/6A in hypertensives LVMI higher than 5A/6A and 5A/5A though males and females. When Univariate Stepwise Regression analysis was used,we found there increased risk for left ventricular remodling associated with course,pulse pressure and genotype 6A/6A in hypertensive patients than controls. Conclusion These results indicate that there was differences in association of MMP-3 5A/6A polymorphism with left ventricular hypertrophy.%目的 探讨基质金属蛋白酶-3(MMP-3)基因启动子-1171 (5A/6A)多态性对高血压患者左室重构的影响.方法 采用限制性片段长度多态性分析技术,检测104例高血压病患者和36例对照者的MMP-3基因启动子-1171 (5A/6A)基因型的表达,并且对并且对104例高血压患者和46例正常人进行超声心动图检查,计算左室重量(LVM)及左室重量指数(LVMI).结果 高血压病组与对照组MMP-3基因启动子-1171 (5A/6A)基因型分布比较差异无统计学意义.高血压病组的LVM、LVMI水平与对照组比较,差异均有统计学意义(P＜0.05).高血压病组内6A/6A基因型者的LVM、LVMI水平较5A/6A和5A/5A型高血压病患者升高,且两组女性患者LVMI水平比较差异有统计学意义(P＜0.05).多因素方差分析及多元逐步回归分析显示,LVMI与患者高血压病程、脉压、6A/6A基因型等均有不同程度的相关.结论 MMP-3基因启动子-1171 (5A/6A)多态性对高血压病患者左室重构有影响.
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