目的：建立豚鼠形觉剥夺超高度近视模型并观察其后极部各层组织的病理组织学变化。方法2周龄三色豚鼠分为形觉剥夺组（12只）和非形觉剥夺对照组（8只），于形觉剥夺前，形觉剥夺后4、6、10、14周分别对各组进行检影和眼轴性参数测量。通过病理组织学光镜检查分析形觉剥夺14周后各组视网膜、脉络膜和巩膜厚度及其形态学的变化。结果豚鼠形觉剥夺后随时间延长近视度数逐渐增高，10周后可达-10.00 D以上的超高度近视，14周后近视度数更高、个别个体可达-20.00 D。眼轴性参数相应延长。形觉剥夺超高度近视眼视网膜、脉络膜和巩膜较对照组均明显变薄并有病理性改变。结论应用遮盖法对豚鼠施行长期单眼形觉剥夺会形成超高度近视，10周后可达-10.00 D以上，超高度近视眼巩膜、脉络膜和视网膜明显变薄，脉络膜和巩膜结构发生紊乱。视网膜结构中感光细胞层变薄最为明显，推测其对超高度近视的发生起重要作用。%Objective To establish an animal model of form-deprived super-high myopia in guinea pigs and to in-vestigate the fundus histopathological changes .Methods Twenty 2-week-old guinea pigs were randomized into form-de-prived group ( n =12) and control group ( n =8).Refraction and axial length were measured before form deprivation , and at 4, 6, 10 and 14 weeks, respectively.The thickness and morphological changes of retina , choroid and sclera were examined with light microscope after 14 weeks.Results The degree of myopia gradually increased after form deprivation , reached -10.00 D after 10 weeks and exceeded -20.00 D after 14 weeks.Axial length increased accordingly .The reti-na, choroid and sclera were obviously thinner with characteristic pathological changes in form -deprived group than those in control group.Conclusion Super-high myopia in guinea pigs could be successfully induced by long-term monocular form deprivation, reaching more than -10.00 D after 10 weeks.The sclera, choroid and retina in super-high myopia eyes be-came much thinner with characteristic structural changes in sclera and choroid .The significantly thinning of photoreceptor layer in retina might play an important role in super-high myopia .
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