目的 通过体外实验探讨转化生长因子β1(TGF-β1)对肠道上皮-间质转分化(EMT)的影响.方法 以浓度为10 ng/ml的TGF-β1刺激肠道上皮细胞株DLD 1,分别在24、48或72 h时,提取细胞总RNA和总蛋白;分别用real-time PCR和Western-blot法检测细胞E-钙黏蛋白(E-cadherin)、N-钙黏蛋白(N-cadherin)、波形蛋白(vimentin)、纤维连接蛋白(fibronectin)、α-平滑肌肌动蛋白(α-SMA)在mRNA和蛋白水平的表达变化.结果 TGF-β1刺激后各个时间点E-cadherin表达显著下降,而N-cadherin、vimentin、fibronectin、α-SMA的表达则明显上调,差异均有统计学意义(P ＜ 0.05).结论 TGF-β1可促进肠上皮细胞EMT过程,可能参与肠道纤维化发病机制.%Objective To investigate the effecicency of transforming growth factor-|31 (TGF-|31) inducing epithelial mesenchymal transition (EMT) in intestinal epithelial cell in vitro. Methods The DLD-1 cells were stimulated with 10 ng/ml TGF-|31 for 24, 48 and 72 hours. Real-time PCR and Western-blot analysis demonstrated TGF-|31 mediated expression of E-cadherin, N-cadherin, α-smooth muscle actin (α-SMA) , fibronectin, and vimentin at the indicated time. Results TGF-β1 mediated repression of E-cadherin, and induction of N-cadherin, a-SMA, fibronectin, and vimentin significantly (P < 0.05). Conclusions TGF-|31 may be involved in the pathogenesis of intestinal fibrosis through promoting the process of EMT.
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