Objective To study the changes and clinical significance of serum levels of matrix metalloproteinase-9 and its inhibitor in newborns with intracranial hemorrhage. Methods Thirty neonates with intracranial haemorrhage were enrolled in the study and 30 healthy neonates were studied as control. Blood samples were collected at 1 d, 3d, 7 d, 15 d after onset, and serum levels of matrix metalloproteinase (MMP)-9 and tissue inhibitor of metalloproteinases (TIMP)-l were measured by enzyme linked immunosorbent assay (ELISA). Results Levels of MMP-9, TIMP-1 and MMP-9/TIMP-1 were significantly higher in the patient group than those in control (P < 0.01). Levels of MMP-9, TIMP-1 and MMP-9/TIMP-1 were highest on 3 d, but began to descend on 7 d. With the recovery of pathogenetic condition, the levels of MMP-9, TIMP-1 and MMP-9/TIMP-1 descended slowly, and recovered normal on 15 d in part of the children, Nevertheless, compared to the controls, these three indexes still remained statistically significant (P < 0.05). In comparison with the controls, levels of MMP-9, TIMP-1 and MMP-9/TIMP-1 in small, medium and large intracranial hemorrhage patients were significantly higher (P < 0.01). The difference of three indexes between these three groups were also significant (P < 0.01). Conclusions Serum levels of MMP-9, TIMP-1 and MMP-9/ TIMP-1 increases obviously in newborns with intracranial hemorrhage, suggesting that they might be used as reference index in assessing pathogenetic condition and appraising prognosis.%目的 探讨经皮测量胆红素浓度对新生儿黄疸检测的准确性和实用性.方法 对400例高胆红素血症新生儿同时进行了经皮测量总胆红素和血清总胆红素的检测,分析两者的相关性,并评估体质量、胎龄、生后日龄、相关病因等对新生儿胆红素水平的影响.结果 血清总胆红素<257 μmol/L时,经皮测量总胆红素与血清总胆红素值的相关系数是0.932,血清总胆红素≥257 μmol/L时,相关系数是0.282,两组均呈正相关性,血清总胆红素<257 μmol/L组相关性高(P=0.0001),且不受胎龄、体质量、生后日龄及相关病因的影响.结论 当胆红素浓度<257 μmol/L,经皮测量总胆红素可以替代血清总胆红素;经皮测量总胆红素用于新生儿高胆的筛查,有可推广性及实用性.
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