目的 检测亚甲蓝病毒灭活血浆中人细小病毒(human parvovirus, HPV) B19抗原和抗体,评估临床输注的安全性.方法 随机抽取2016年2月5日～4月30日制备的亚甲蓝病毒灭活血浆1056袋,采用ELISA法检测HPV B19抗原和抗体;将HPV B19抗体阳性血浆按献血者性别、年龄、血型进行分组统计分析.结果 1 056袋亚甲蓝病毒灭活血浆HPV B19抗原均为阴性,lgG和lgM抗体总阳性率为20.5％ (216/1 056)、IgG抗体阳性率为19.5％ (206/1056)、IgM抗体阳性率为4.9％ (52/1056),不同来源献血者制备的血浆HPV B19抗体阳性率有差异,随年龄增加抗体阳性率呈上升趋势,献血者性别差异无统计学意义,A型和AB型抗体阳性率较高,B型和O型抗体阳性率较低.结论 亚甲蓝病毒灭活血浆中HPV B19抗原、抗体检测结果显示,临床输注血浆感染HPV B19的风险不高,但对于免疫缺陷等特殊受血者应引起重视,建议进行输血前HPV B19检测.%Objective To detect and analyze the human parvovirus B19 antigens and antibodies in methylene blue inactivated virus-free plasma to evaluate the safety of blood transfusion. Method 1 056 bags of plasma inactivated with methylene blue were randomly selected and prepared from February to April, 2016, for detection of the human parvovirus B19 antigens and antibodies using ELISA. Samples of the positive HPV B19 antibodies were grouped for statistical analyses according to gender, age, and blood groups. Results Parvovirus B19 virus antigens were negative in all 1056 bags of methylene blue inactivated virus-free plasma, whereas IgG and IgM antibodies were positive with the overall prevalence of20.5％ (216/1056), with 19.5％ (206/1056) IgG positive rate being, and 4.9％ (52/1056) IgM positive rate. The HPV B19 antibody positive rate were found to be different among different blood donors: B19 positive rate increased with age, antibodies to B19 in blood group A and AB was higher than in groups B and O.No statistically significant difference was noted in gender. Conclusion Although the risk of Yichang residents at human parvovirus B19 infection from blood transfusion is not high, detection of HPV B19 before blood transfusion is significant for some recipients such as immunocompromised patients.
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