目的：检测C－met mRNA和C－src mRNA在甲状腺乳头状癌（ thyroid papillary cancer，TPC）中的表达并探讨其与临床病理特征的关系。方法采用半定量逆转录聚合酶链反应（ RT－PCR）方法检测46例甲状腺乳头状癌和18例良性病变中C－met mRNA和C－src mRNA的表达，并分析二者表达的相关性及其与临床病理特征的关系。结果 C－met mRNA和C－src mRNA在TPC中的表达量分别为1．140±0．163和0．984±0．187，在甲状腺良性病变的表达量分别为0．467±0．237和0．484±0．254，二者在TPC与甲状腺良性病变的表达差异均有显著性意义（P＜0．001，P＜0．001）。 TPC组织C－met mRNA的表达量与患者年龄有关（P＜0．05）；TPC组织C－src mRNA的表达量与肿瘤大小及淋巴结转移有关（均P＜0．05）。TPC中C－src mRNA和C－met mRNA的表达量呈正相关（r＝0．764，P＜0．001）。结论 TPC存在C－src mRNA和C－met mRNA的异常表达且C－src mRNA可能能为TPC的淋巴结转移提供依据。%Objective To study the expressions of C-src mRNA and C-met mRNA in thyroid papillary cancer ( TPC) and to analyze the relationship between their expressions and clinicopathological features.Methods Reverse transcription polymerase chain reaction ( RT-PCR) technique was used to examine the expressions of C-src mRNA and C-met mR-NA in 46 TPCs and 18 benign thyroid tissues.The relationship between their expressions and clinicopathological features was analyzed.Results C-met mRNA and C-src mRNA expression levels in 46 TPCs were 1.140 ±0.163 and 0.984 ± 0.187 respectively.Both were significantly higher than benign thyroid tissues (P<0.001, P<0.001).C-src mRNA ex-pression in TPC was associated with tumor grades and lymph node metastasis (P<0.05, P<0.05).C-met mRNA ex-pression has an ascending trend with aging (P<0.05).C-src mRNA expression closely related to C-met mRNA expres-sion (r=0.764, P<0.001).Conclusion The abnormal expressions of C-src mRNA and C-met mRNA could play a role in the carcinogenesis and development of TPC.
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