利用微生物Absidia sp.R9g产的芦丁-α-鼠李糖苷酶(RhaR)转化芦丁制备异槲皮苷,采用硅胶柱层析法对酶解产物进行分离纯化.结果表明,24.0 g芦丁的酶解产物,经分离可得到较纯的异槲皮苷单体13.1g,其得率为49.4％.通过高效液相色谱检测其纯度达到98.3％.%Isoquercitrin was transformation from rutin by rutirrarrhamnosidase (RhaR) produced with Absidia sp. R9g. Hydrolysate was separated from 24. 0 g rutin by silica gel column and 13. 1 g isoquercitrin was obtained. The yield was 49. 4% and the purity of the product was 98. 3%.
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