本研究观察干扰素γ(IFN-γ)对体外培养人脐带间充质基质细胞(UC-MSC)表面黏附分子表达水平的影响.采用组织块移行法培养人UC-MSC,并进行细胞表面抗原、成骨和成脂鉴定.向第3代UC-MSC加入不同浓度的IFN-γ,干预24h后收集细胞,应用流式细胞仪检测CD54、CD58、CD62p、CD62L、CD44、CD49d、CD102及CD106黏附分子的表达水平.结果表明,生理状态下,CD106、CD62P、CD62L和CD102阳性表达率极低(均＜1％),CD54表达最高(41.58±0.83)％；经IFN-γ干预后,CD102、CD106、CD62L、CD62p阳性表达率略有升高,但总体变化不明显(均＜5％)；CD54、CD58阳性表达率与IFN-γ呈浓度依赖性,最高达(59.66±1.36)％,(43.96±0.62)％；CD49d的阳性表达率在100 U/ml时达到峰值(51.33±0.74)％,CD44在浓度为1 000 U/ml时阳性表达率最高(73.22±1.93)％.结论:IFN-γ可显著提高UC-MSC表面CD54、CD58、CD44、CD49d的阳性表达率,但对CD102、CD106、CD62P和CD62L作用不明显.%This study was purposed to investigate the effects of interferon (IFN) -y on expression of adhesion molecules in mesenchymal stromal cells derived from human umbilical cord tissue (UC-MSC). The UC-MSC were isolated from human umbilical cord by tissue culture. The expressions of specific markers on UC-MSC were detected by flow cytometry in the physiological condition. The adipogenic and osteogenic induction of UC-MSC was detected by alizarin and Oil red O staining. UC-MSC were exposed to IFN-7 (100, 1 000, 10 000 U/ml) for 24 h, the expressions of CD54, CD58, CD44, CD49d, CD62p, CD62L, CD102 and CD106 on cell surface were detected using flow cytometry. The results showed that in physiological condition, UC-MSC extremely low expressed CD102, CD106, CD62P, CD62L, while the expression of CD54 was relatively high(41.58 ±0. 83)% . When stimulated by IFN-γ, the expression of CD102, CD106, CD62P, CD62L increased slightly, but still low ( < 5% ), while CD54 and CD58 upregulated concentration-dependently up to (59. 66 ± 1.36) % and (43. 96 ± 0. 62) % respectively. The expression of CD49d upregulated to (51. 33 ±0. 74)% when UC-MSC exposed to IFN-7 100 U/ml. CD44 increased to (73. 22 ± 1.93)% when UC-MSC exposed to IFN-7 1 000 U/ml. It is concluded that IFN-γ can elevate significantly the expression of CD54, CD49d, CD44 and CD58, but has no significant effect on CD102, CD106, CD62P and CD62L expression on the surface of UC-MSC.
展开▼
