首页> 中文期刊> 《中国水产科学》 >栉孔扇贝、虾夷扇贝及其杂交子代的群体遗传多样性分析

栉孔扇贝、虾夷扇贝及其杂交子代的群体遗传多样性分析

         

摘要

Amplified fragment length polymorphism (AFLP) technology was used to analyze the genetic variation and genetic structure among three populations of Chlamys farreri(♀), Patinopecten yessoensis(♂)and their offspring F1. In total, 315 loci were amplified with five primer pairs, and 311 of these loci were polymorphic, accounting for 98.73%. Shannon genetic diversity indices were 0.477 1±0.260 6, 0.423 9±0.277 7 and 0.548 8±0.220 9, respectively for the female parent, the male parent and F1, while Nei's gene diversities were 0.329 1±0.185 0, 0.289 7±0.195 9 and 0.382 8±0.161 2, respectively. The genetic distance between C. farreri and P. yessoensis was 0.411 6, while it was 0.131 6 between F1 and their female parents, and 0.278 0 between F1 and their male parents. The dendrograms showed that the three population gathered independently, which indicated that each population was independent. AMOVA analysis indicated that 30.47% of variance was among populations and 69.53% variance was within populations, which suggested rich genetic diversity level within populations. In all, the expression of heterosis has a correlation with the genetic variation.%采用扩增片段长度多态性(Amplified Fragment Length Polymorphism,AFLP)标记对栉孔扇贝(Chlamys farreri,♀)、虾夷扇贝(Patinopecten yessoensis,♂)及其Fl群体的遗传多样性进行分析.5对引物组合共扩增得到315个位点,其中311个为多态位点,总的多态位点比例为98.73%.栉孔扇贝、虾夷扇贝及其F1群体的Shannon信息指数分别为0.477 1±0.260 6、0.423 9±0.277 7、0.548 8±0.220 9;3个群体的Nei's基因多样性指数分别为0.329 1±0.185 0、0.289 7±0.195 9、0.382 8±0.161 2,表明杂交子代群体的遗传多样性水平比2个亲本群体都高.栉孔扇贝与虾夷扇贝群体间的遗传距离为0.411 6,栉孔扇贝与F1群体间的遗传距离是0.131 6,虾夷扇贝与F1群体间的遗传距离是0.278 0,表明杂种子代与母本栉孔扇贝遗传距离较近.聚类分析显示,虾夷扇贝群体、栉孔扇贝群体以及F1群体的所有个体分别聚到一起,且都形成独立群体;分子方差分析(AMOVA)结果显示,变异来源有30.47%来自群体间,有69.53%来自群体内,表明群体内的遗传多样性比较丰富.杂种优势的产生与群体遗传多态性有关.

著录项

  • 来源
    《中国水产科学》 |2011年第3期|574-580|共7页
  • 作者单位

    农业部海洋渔业资源可持续利用重点开放实验室,中国水产科学研究院黄海水产研究所,山东青岛266071;

    上海海洋大学水产与生命学院,上海201306;

    农业部海洋渔业资源可持续利用重点开放实验室,中国水产科学研究院黄海水产研究所,山东青岛266071;

    农业部海洋渔业资源可持续利用重点开放实验室,中国水产科学研究院黄海水产研究所,山东青岛266071;

    农业部海洋渔业资源可持续利用重点开放实验室,中国水产科学研究院黄海水产研究所,山东青岛266071;

    农业部海洋渔业资源可持续利用重点开放实验室,中国水产科学研究院黄海水产研究所,山东青岛266071;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 水产生物学;
  • 关键词

    栉孔扇贝; 虾夷扇贝; F1; AFLP; 遗传多样性; 杂种优势;

相似文献

  • 中文文献
  • 外文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号