Objective: To setup the rapid inspection method for detecting and evaluating PTEN, VEGF and RRM1 mRNA expression in non-smal cel lung cancer tissues. Method:Peripheral blood from normal people is used to set up the standard curves of PTEN, VEGF,RRM1 and actin (housekeeping gene) expression, then double-standard curves method of relative quantification PCR is employed to detect gene expression of 80 NSCLC and 200 normal tissue samples. Result: The PTEN gene expression level was significantly higher in NSCLC than that in normal tissues. The VEGF expression level, however, was significantly lower in NSCLC compared with that in normal tissues. PTEN and VEGF gene expression levels did not correlate with sex and histological types but correlate with the p-TNM stages in a negative manner. RRM1 expression did not dependent on sex, histological types, and p-TNM stages. Conclusion:Taqman real-time fluorescent quantitative PCR could be employed to evaluate the expression levels of PTEN, VEGF and RRM1 in NSCLC patients, and also as a reliable reference for treatment and therapy.% 目的建立快速检测非小细胞肺癌患者PTEN、VEGF和RRM1 mRNA表达水平的体系,并对表达水平进行评价.方法以正常人外周血构建PTEN、VEGF、RRM1和管家基因actin的定量标准曲线,利用实时荧光定量PCR法中的“双标准曲线”模型对80例肺癌患者和200例正常人的上述基因表达水平进行检测.结果标准曲线呈良好的线性关系.PTEN和VEGF基因在非小细胞肺癌组织中表达分别显著高于和低于正常组织,PTEN、VEGF基因表达与患者的性别、组织学类型无关,而与P-TNM分期存在显著相关性.两者的基因表达呈负相关.RRM1基因表达与患者的性别、组织学类型以及P-TNM分期均不存在显著相关性.结论 Taqman实时荧光定量PCR法能够较好的对非小细胞肺癌患者体内PTEN、VEGF和RRM1 mRNA表达水平进行定量检测并给予客观评价,为后期的治疗和用药提供可靠的依据.
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