A hybrid heterokaryon, H15-21 fruiting, was abtained by mating two single spore isolates non-fruiting, PYd21 and FYdl5. The genomes of PY21 and PYd15 were sequenced by de novo sequencing and re-sequencing respectively. Triosephate isomerase (TPI) genes were cloned and sequenced from both PYd21 and PYd15. Meanwhile their structures were analyzed by tran-scriptional reads mapping and paired-end analysis. Alignment analysis revealed that both sequences of TPI gene from both isolates were identical. The 77V gene was consisted of 1015 bp with an open reading frame of 756 bp encoding a polypepn'de of 251 amino acids. TPI gene contained 6 exons and 5 introns. Two types of alternative splicing and two alternative splicing sites were identified during RNA processing. TPM (number of tags mapped only from TPI x l06/total number of tags) values for PYdl2, PYdl5 and H15-21 were 30.25, 36.49 and 77.17 respectively, which indicated that the two nuclei (from PYd21 andPYdl5) in H15-21 interacted synergistically with each other. Real-time quantitative PCR used to measure TPI gene expression levels in the three strains, confirmed this effect.%分别从不能出菇的草菇单孢菌株PYd21(基因组测序菌株)和PYd15(重测序菌株)及由PYd21、PYd15配对杂交获得可正常出菇的异核菌株H15-21中克隆测序了磷酸丙糖异构酶(TPI)基因,并对TPI基因的结构及其在3个菌株中的表达量进行了分析.结果表明:PYd21、PYd15中TPI基因的序列完全相同；转录组读段定位、双末端分析结果显示,TPI基因全长1015 bp,开放阅读框序列全长756 bp,编码251个氨基酸,有6个外显子、5个内含子；RNA加工过程中存在两种可变剪切类型和两个可变剪切位点；数字基因表达谱测序的结果表明,PYd21、PYd15和H15-21中TPI基因的表达量(TPM)分别为30.25、36.49、77.17,同时通过实时荧光定量PCR分析3个菌株中TPI基因的表达情况证实了该结果,表明草菇中TPI基因的表达表现出异核体表达的协同增效作用,预示着异核菌株中两种不同细胞核存在相互作用.
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