Effects of temperature and time on caspase-9 activity and DNA in genome of adult diamondback moth (DBM) was studied. The caspase-9 enzyme activity increased significantly when the adult DBM was pretreated with high temperature (40, 42, 45,47 ℃ ) in short time. Heat stress could cause DNA fragmentation, and the apoptotic DNA ladder fragments could be visualized by agarose gel. These results suggested that heat stress could induce apoptosis in DBM.%研究了不同温度下不同时间处理对小菜蛾caspase-9蛋白酶活性和基因组DNA的影响.结果表明,40、42、45、47℃4个温度处理均能在短时间内使caspase-9蛋白酶活性显著增强;此外,33.5和40℃处理72 h可导致小菜蛾基因组DNA显著降解,电泳时呈现细胞凋亡的特征条带.高温能够诱导小菜蛾细胞凋亡.
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