以苋菜种子为材料,进行苋菜离体繁殖研究.结果表明:苋菜种子经过75％酒精消毒1 min,再用升汞消毒8 min,在MS培养基上的萌发率可达87％以上；从无菌苗切取带2片子叶的下胚轴,在MS +3.0 mg·L-1 6-BA +0.2 mg·L-1 NAA培养基上诱导不定芽的效果最好；不定芽在MS +3.0 mg·L-1 6-BA+ 1.0 mg·L-1 IAA培养基上的增殖效果最好,增殖系数可达13.02；试管苗在1/2MS +0.2 mg·L-1 IBA培养基上的生根效果较好,生根率可达90.35％；在适宜的温度下,试管苗移栽到菜园土上的成活率可达92.5％以上.%Seeds were used to study in vitro propagation of amaranth (Amaranth mangostanus L. ). The results showed that the germination rate of seeds reached 87% or higher in the MS medium after they were disinfected with in 75% alcohol lmin and in 0.1% mercuric chloride 8 min. The best medium for adventitious bud induction of hypocotyl with two cotyledons was MS with addition of 3.0 mg · L-1 6-BA, 0.2 mg · L-1 NAA, for propagation was MS with addition of 3.0 mg · L-1 6-BA, 1.0 mg · L-1 IAA, by which the proliferation coefficient was up to 13.02, for rooting was 1/2MS with addition of 0.2 mg · L-1 IBA, by which the rooting rate was 90.33%. When the plantlets were transferred into the garden soil, the survival rate was up to 92.5% at fit temperature.
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