Bacillus thuringiensis strain BRC-HZP7 was isolated from the soil of Wuyi mountain, which exhibited the high activity a-gainst Plutella xylostella larvae and Spodoptera exigua larvae. Biochemical reactions were tested for its biological characters. The cry gene types and their encoded proteins of BRC-HZP7 were identified by the methods of PCR-RFLP, SDS-PAGE and PMF. The results showed that cry1Aa, crylBa, cry1Ia, cry2Ab, cry2Ac and cry2Ae existed in BRC-HZP7. Eight interesting protein bands were picked out to analyze further by MALDI-TOF/MS. PMF matching analysis results showed that band 3,6,7,8 were ICPs. The band 6,7,8 were identified as Cry2Aa, Cry1Aa, Cry1Ba, respectively. The band 3 was possible partial fragment of Cry1Ba. The strain with abundant ICPs would be a good resource in pest biocontrol.%分离自武夷山土壤的苏云金芽胞杆菌BRC-HZP7菌株对小菜蛾和甜菜夜蛾幼虫有较好的毒杀作用.对该菌株进行了生理生化测定、cry基因的PCR-RFLP检测,以及通过SDS-PAGE检测晶体蛋白,并对蛋白点进行肽段指纹图谱鉴定.结果表明该菌株含有cry1Aa、cry1 Ba、cry1 Ia、cry2Ab、cry2Ac、cry2Ae等6种cry基因.从选取的8个蛋白条带肽段指纹图谱可以看出:标记3、6、7、8的4个蛋白点都是杀虫晶体蛋白,蛋白点6、7、8分别鉴定为Cry2Aa、Cry1 Aa、Cry1 Ba,蛋白点3可能是Cry1Ba的部分片段.表明该菌株有丰富的ICP资源,可作为杀虫应用的候选菌株.
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