[ABSTRACT]Objective:To study the relationship of serum human epididymis protein (HE4),cancer protein-2 (CP2)and human cartilage glycoprotein-39 (HCgp-39)levels with tumor malignancy in patients with endometrial carcinoma.Methods:A total of 90 patients with endometrial carcinoma treated in our hospital between May 2012 and August 201 5 were collected and divided into early-to-mid (Ⅰ-Ⅲ stage)endometrial carcinoma group (n =59)and advanced (Ⅳ stage)endometrial carcinoma group (n =31)according to the pathological staging,and 34 patients with endometrial thickening who received uterine curet-tage in our hospital during the same period were selected as control group.The levels of HE4,CP2,HCgp-39 and tumor mark-ers in serum as well as the mRNA expression of proliferation genes and invasion genes in tumor tissue were determined.Re-sults:HE4,CP2,HCgp-39,CA125,CA1 9-9 and CEA levels in serum as well as Bcl2,Chk1,PIK1,HER2 and GDF-1 5 mR-NA expression in tumor tissues of early-to-mid endometrial carcinoma group and advanced endometrial carcinoma group were significantly higher than those of control group while the miRNA-1 99a-3p,Bax,caspase3,BRCA1,Kiss-1 and KAI1 mRNA expression were lower than those of control group;HE4,CP2,HCgp-39,CA125,CA1 9-9 and CEA levels in serum as well as Bcl2,Chk1,PIK1,HER2 and GDF-1 5 mRNA expression in tumor tissues of advanced endometrial carcinoma group were sig-nificantly higher than those of early-to-mid endometrial carcinoma group while the miRNA-1 99a-3p,Bax,caspase3,BRCA1, Kiss-1 and KAI1 mRNA expression were lower than those of early-to-mid endometrial carcinoma group;serum HE4,CP2 and HCgp-39 levels were positively correlated with CA125,CA1 9-9,CEA,Bcl2,Chk1,PIK1,HER2 and GDF-1 5,and negatively correlated with miRNA-1 99a-3p,Bax,caspase3,BRCA1,Kiss-1 and KAI1.Conclusions:Serum HE4,CP2 and HCgp-39 lev-els can directly reflect the tumor malignancy in patients with endometrial carcinoma,and are expected to become the reliable means for tumor screening and treatment guidelines.%目的：：探讨子宫内膜癌患者血清人附睾蛋白(HE4)、癌蛋白-2(CP2)、人软骨糖蛋白-39(HCgp-39)含量与肿瘤恶性程度的关系。方法：收集2012年5月~2015年8月本院收治的子宫内膜癌患者90例,根据病理分期分为早中期(Ⅰ~Ⅲ期)子宫内膜癌组59例、晚期(Ⅳ期)子宫内膜癌组31例,另取同期在本院接受刮宫治疗的子宫内膜增厚患者34例作为对照组。测定血清中 HE4、CP2、HCgp-39及肿瘤标志物的含量以及肿瘤组织中增殖基因、侵袭基因 mRNA 表达量。结果：早中期子宫内膜癌组、晚期子宫内膜癌组患者血清中 HE4、CP2、HCgp-39、糖类抗原125(CA125)、糖类抗原19-9(CA19-9)、癌胚抗原(CEA)的含量以及肿瘤组织中 Bcl2、Chk1、PIK1、HER2、GDF-15的 mRNA 表达量均显著高于对照组,miRNA-199a-3p、Bax、caspase3、BRCA1、Kiss-1、KAI1的 mRNA 表达量低于对照组；晚期子宫内膜癌组患者血清中 HE4、CP2、HCgp-39、CA125、CA19-9、CEA 的含量以及肿瘤组织中 Bcl2、Chk1、PIK1、HER2、GDF-15的 mRNA 表达量均显著高于早中期子宫内膜癌组,miRNA-199a-3p、Bax、caspase3、BRCA1、Kiss-1、KAI1的 mRNA 表达量低于早中期子宫内膜癌组；血清 HE4、CP2、HCgp-39含量与 CA125、CA19-9、CEA、Bcl2、Chk1、PIK1、HER2、GDF-15呈正相关,与 miRNA-199a-3p、Bax、caspase3、BRCA1、Kiss-1、KAI1呈负相关。结论：子宫内膜癌患者血清 HE4、CP2、HCgp-39含量可直接反映肿瘤恶性程度,有望成为肿瘤筛查及治疗指导的可靠手段。
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