目的:探讨胃癌患者血清中胃蛋白酶原Ⅰ/Ⅱ比值和胃泌素-17含量与癌细胞恶性增殖的相关性.方法:选择2016.8~2017.8月期间收集胃镜活检病理确诊的浅表性胃炎组247例、胃溃疡组159例、胃癌组94例,对比三者血清胃蛋白酶原Ⅰ /Ⅱ比值和胃泌素-17含量,病灶组织中增殖及凋亡基因表达量的差异.采用Pearson检验评估胃癌患者胃蛋白酶原Ⅰ/Ⅱ比值和胃泌素-17含量与癌细胞恶性增殖活性的相关关系.结果:胃癌组血清胃蛋白酶原Ⅰ/Ⅱ比值、胃泌素-17含量低于胃溃疡组、浅表性胃炎组.胃癌组增殖基因 EIF5A2、MACF1、PIK3CD mRNA 表达量高于胃溃疡组、浅表性胃炎组, SIRT1 mRNA表达量低于胃溃疡组、浅表性胃炎组;凋亡基因Livin mRNA 的表达量高于胃溃疡组、浅表性胃炎组,Bad、Noxa mRNA的表达量低于胃溃疡组、浅表性胃炎组;胃癌组血清中胃蛋白酶原Ⅰ /Ⅱ比值、胃泌素-17含量与 EIF5A2、M ACF1、PIK3CD、Livin mRNA 表达量呈负相关,与 SIRT1、Bad、Noxa mRNA表达量呈正相关.结论:胃癌患者血清中胃蛋白酶原Ⅰ /Ⅱ比值和胃泌素-17含量异常降低,且具体水平与胃癌细胞增殖、凋亡活性直接相关.%Objective:To investigate the correlation of serum pepsinogen Ⅰ /Ⅱ ratio and gastrin-17 content with malig-nant proliferation of cancer cells in patients with gastric cancer. Methods:Superficial gastritis group(n=247),gastric ulcer group(n=159)and gastric cancer group(n=97)who were pathologically diagnosed by gastroscopy in Chongqing wanzhou district people's hospital of 5 between August 2016 and August 2017 were selected,and the differences in serum pepsinogen Ⅰ /Ⅱ ratio and gastrin-17 content as well as proliferation and apoptosis gene expression in lesion tissue were compared among the three groups. Pearson test was used to evaluate the correlation of pepsinogen Ⅰ /Ⅱ ratio and gastrin-17 content with malignant proliferation activity of cancer cells in patients with gastric cancer. Results:Serum pepsinogen Ⅰ /Ⅱ ratio and gastrin-17 con-tent in gastric cancer group were lower than those in gastric ulcer group and superficial gastritis group. Proliferation genes EIF5A2,MACF1 and PIK3CD mRNA expression levels in gastric cancer group were higher than those in gastric ulcer group and superficial gastritis group whereas SIRT1 mRNA expression level was lower than that in gastric ulcer group and superficial gastritis group;apoptosis gene Livin mRNA expression level was higher than that in gastric ulcer group and superficial gastritis group whereas Bad and Noxa mRNA expression levels were lower than those in gastric ulcer group and superficial gastritis group;serum pepsinogen Ⅰ /Ⅱ ratio and gastrin-17 content in gastric cancer group were negatively correlated with EIF 5A2, MACF1,PIK3CD and Livin mRNA expression levels,and positively correlated with SIRT 1,Bad and Noxa mRNA expression levels. Conclusion:The serum pepsinogen Ⅰ /Ⅱ ratio and gastrin-17 content abnormally reduce in patients with gastric cancer, and the specific levels are directly correlated with gastric cancer cell proliferation and apoptosis activity.
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