In order to investigate the potential role of ZmNAP in regulating the anti-senescence and yield traits of maize,the maize ZmNAP RNA interference vector pTCK303ihpZmNAP driven by Ubi promoter was created based on the ZmNAP cDNA sequence deposited in MaizeGDB, and was transformed into maize by Agrobacterium tumefaciens. The results showed that transgenic maize plants had more obvious advantages than control plants in delaying senescence and grain weight. The 1 000-grain weight of transgenic maize plants was 15%—30% higher than that of wild type maize plants. ZmNAP gene has potential value in maize breeding.%为了探索ZmNAP基因在调控玉米抗衰老及产量性状等方面的作用,根据已知的ZmNAP基因cDNA序列构建玉米Ubi启动子控制下的ZmNAP的RNA干扰载体pTCK303ihpZmNAP,并通过农杆菌介导的方法对ZmNAP基因进行遗传转化.结果表明:ZmNAP转基因玉米植株在叶片滞绿、籽粒千粒重等方面较野生型植株(WT)有明显优势,转基因植株比野生型植株千粒重提高15％～30％.ZmNAP基因在玉米育种中具有重要的应用价值.
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