Plantlet regeneration system was established with leaves and petioles of Paulownia 9501 and the tetraploid seedlings was identified using counting the number of chromosome of root tip cells of plantlets and DNA content of single cells of mature leaves. The results showed that the leaves of Paulownia 9501 were the best explants, and the optimal culture medium for callus, shoots and roots were MS +0.7 mg· L-1 NAA + 6mg· L-16-BA and 1/2 MS, MS +0.7 mg· L-1 NAA +8 mg· L-1 6 - BA and 1/2 MS respectively. The highest rate of induced autotetraploid was 18.57％ when the leaves of pretreatment of 8 d were treated for 72 h with concentration of 10 mg · L-1 by colchicine. The leaves of autotetraploid increased and thickened and its stomata grew larger and stomatal density was smiler than diploid.%以泡桐9501无菌苗的叶片、叶柄为外植体,建立了其体外植株再生系统.在此基础上进行了四倍体诱导,并采用根尖染色体计数和成熟叶片单细胞的DNA含量测定进行诱变植株的倍性分析.结果表明,泡桐9501叶片为最佳外植体,其愈伤组织、芽和根诱导的最适培养分别为MS+0.7 mg·L-1NAA+6 mg·L-1 6-BA和1/2MS,MS+0.7 mg·L-1 NAA+8 mg·L-16-BA和1/2MS.在秋水仙素质量浓度为10mg·L-1处理预培养8 d泡桐叶片72 h时,四倍体诱导率最高达18.57%.诱导出的四倍体植株叶片较二倍体增大、增厚,叶片单个气孔器变大,叶片气孔密度变小.
展开▼
