采用DA -201大孔吸附树脂从芝麻混合油中分离纯化芝麻素,优化得到最佳工艺条件为:上样液中芝麻素浓度3.0 mg/mL左右,吸附流速3.0 BV/h；解吸液为体积分数95％的乙醇,解吸流速3.0 BV/h,解吸液用量8.0 BV；在此条件下,芝麻素回收率为85.21％,分离得到的芝麻素浓缩物中芝麻素含量为13.00％,与芝麻原油相比,纯度提高了近12倍.芝麻素浓缩物经过结晶纯化,得到最终产品中芝麻素的总回收率为62.06％,产品纯度为89.49％.%In this paper,we determined the optimized processing conditions for separating and purifying sesa-min from mixed sesame oil by using macroporous adsorption resin DA-201. The optimized conditions were as follows:the concentration of sesamin in loading solution was about 3.0 mg/mL,and the adsorption flow rate was 3. 0 BV/h;95% ethanol was used as desorption solvent;and the desorption rate and the amount of the de-sorption solvent were 3. 0 BV/h and 8. 0 BV respectively. Under the optimized conditions, the recovery rate of sesamin reached 85.21% ,and the yield of seamin was 13% , so that the purity of sesamin was improved by nearly 12 times in comparison with crude sesame oil. The total recovery rate and the purity of sesamin in the final product obtained by crystallizing the sesamin concentrate were 62. 02% and 89. 49% ,respectively.
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