Objective To study the expression of RANK, RANKL, caspase-1, IL-18, IL-33 and BAFF-R in RA patients.Methods 72 RA patients were selected and 70 healthy check-ups were selected as control group. The level of RANK, RANKL, caspase-1, IL-18, IL-33 and BAFF-R for RA group and control group was detected. Results ①The expression rate of RANK and RANKL for RA group and control group was not significantly different; the expression intensity of RANK in CD3+T lym-phocyte cell, CD14+monocyte and neutrophile granulocyte for RA group was significantly lower than that of control group ; the expression intensity of RANKL for RA group and control group was not significantly different ; ②The level of caspase-1, IL-18, IL-33 for RA group was significantly higher than that of control group ; according to the correlation analysis, the expression of caspase-1 was positively correlated with the expression of IL-18 and IL-33; ③ The level of BAFF-R for RA group was sig-nificantly higher than that of control group. Conclusion For RA patients, the detection of RANK, RANKL, caspase-1, IL-18, IL-33 and BAFF-R can make a comprehensive evaluation on the illness progress. In addition, the genesis and development of RA is contributed to the concurrent action of multiple mechanisms.%目的:探讨类风湿关节炎(RA)患者核因子 κB受体活化因子(RANK)及其配体(RANKL)、半胱氨酸蛋白酶-1(caspase-1)、白介素-18(IL-18)、IL-33及B淋巴细胞表面表达的B淋巴细胞刺激因子受体(BAFF-R)水平表达情况.方法:临床纳入RA患者72例,同时纳入70例健康体检者作为研究的对照组.分别检测RA组患者以及对照组外周血RANK、RANKL、caspase-1、IL-18、IL-33及BAFF-R等水平.结果:①RA组RANK、RANKL表达率差异无显著性;但RA组RANK在CD3+T淋巴细胞、CD14+单核细胞、中性粒细胞上的表达强度要明显低于对照组,差异有显著性;RA组与对照组RANKL表达强度差异无显著性;②RA组caspase-1及其下游因子IL-18、IL-33水平均明显高于对照组,差异有显著性;相关性分析显示,caspase-1与IL-18、IL-33呈现正相关;③RA组BAFF-R水平明显高于对照组,差异有显著性.结论:通过对RA患者进行外周血RANK、RANKL、caspase-1、IL-18、IL-33及BAFF-R水平检测,能够综合评估病情的进展.另外,RA的发生及发展是多种机制共同作用的结果.
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