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首页> 中文期刊> 《湖南中医药大学学报》 >愈痫灵方及其加味方对戊四氮致痫鼠海马组织中TLR4、MyD88表达的影响

愈痫灵方及其加味方对戊四氮致痫鼠海马组织中TLR4、MyD88表达的影响

         
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目的：探讨愈痫灵方及其加味方对痫性大鼠海马组织中 TOLL 样受体4（ toll-like receptor4， TLR4）与髓样分化蛋白D88（ myeloid differentiation factor 88， MyD88）的影响。方法取健康雄性 SD 大鼠130只，从中随机选取10只作为正常对照组；选取10只作为假手术组，腹腔注射生理盐水；其余大鼠用戊四氮造模，将符合模型标准的大鼠随机分为模型组、丙戊酸钠组、愈痫灵方组、愈痫灵加银花组，每组10只。灌胃后断头取脑，采用逆转录荧光实时定量聚合酶链反应（ reverse transcription PCR ， RT-PCR ）检测各组大鼠海马中 TLR4 mRNA 的表达，免疫组化法检测各组大鼠海马中 CA3区MyD88蛋白的表达。结果 TLR4 mRNA 和MyD88表达，模型组与正常对照组、假手术组比较差异有显著统计学意义（ P<0.01）；愈痫灵方组、愈痫灵方加银花组、丙戊酸钠组分别与模型组比较差异有显著统计学意义（P<0.01）；愈痫灵方组和愈痫灵方加银花组分别与丙戊酸钠组比较差异有统计学意义（P<0.05）。结论海马组织中 TLR4 mRNA、MyD88表达的增多在癫痫发生发展过程中具有很重要的作用，愈痫灵方可能通过下调致痫鼠海马中 TLR4 mRNA、MyD88的表达来改善痫性大鼠海马损伤程度。%〔Abstract〕 Objective To explore the influence of Yuxianling and its modified decoction on the expressions of Toll-like receptor 4 (TLR-4) and the myeloid differentiation factor 88 (MyD88) in hippocampus of epileptic rats. Methods The 130 male healthy Sprague-Dawley rats were randomly divided into control group (n=10), sham-operation group (n=10), pentylenetetrazole (PTZ) group (n=110). The sham operation group were injected with equivalent saline, and the rats of PTZ group were intraperitoneal injected with pentylenetetrazole. The standard epileptic rats were randomly divided into model group, valproate group, Yuxianling decoction (YXL) group, modified Yuxianling decoction (MYXL) group, 10 rats in each group. The expression of TLR4 mRNA protein in rat hippocampus were detected by RT-PCR, and the expression of MyD88 protein was tested by immunohistochemical method.Results The expression of TLR4 mRNA and MyD88 protein in model group was significantly higher than control group and sham-operation group (P<0.01), the expressions in YXL, MYXL and valproate groups comparing with the model group were statistically significant (P<0.01), the expressions in YXL and MYXL groups comparing with the valproate group were statistically significant There was no significant differences between control group and sham-operation group (P>0.05); the expression of TLR4 mRNA and MyD88 protein in YXL and YXLH was much weaker than that invalproate group (P <0.05). Conclusion The increased expression of TLR4 mRNA and MyD88 protein in epileptic rats hippocampus may play an promotion role on the development of the hippocampus injury. Yuxianling decoction can down regulate the expression of TLR4 mRNA and MyD88 protein and lessen the pathologic changes of hippocampus.

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