Objective:Ulipristal acetate (UPA),a progesterone receptor modulator,has been used as an emergency contraceptive.In this study,we investigated the effect of UPA on human sperm parametersby in vitro experiments.Methods:Fifteen semen samples with normal parameters were used.After liquidation and resuspend with G-IVF,each sample was sub-divided into 6 groups,0.5 mL each sample.Spermatozoa were incubated in the culture medium containing 0.04,0.4,4 and 40 μmol/L of UPA.Two controls were the blank control and the DMSO control.The sperm viability,motility,DNA integrity,acrosome reaction,sperm hyperactivation,and intracellular free calcium concentration were measured.Results:There were no significant differences in the viability,motility and morphology among two controls and four treatment groups (P＞0.05).In those groups treated with UPA at greater concentration than 0.4 μmol/L,the ratio of DNA damage and tail moment were significantly increase,the ratio of sperm hyperactivation was reduced,and the acrosome reaction and the calcium ion concentration in spermatozoa were significantly reduced (P＜0.01).Conclusions:In the in vitro experiments,we found that UPA inhibited the acrosome reaction and hyperactivation of human sperm,and decreased the concentration of intracellular calcium.These actions could be related to the sperm damage induced by UPA.%目的:醋酸乌利司他(ulipristal acetate,UPA)是一种孕激素受体调节剂,目前作为紧急避孕药物广泛应用于临床.本研究探索在体外UPA对人精子参数与功能的影响.方法:选择15例精液参数正常的标本,液化后以G-IVF调节精子浓度为2×106/mL.每例标本再分为6份,每份0.5 mL,共6组(n=15).密度梯度离心后,其中4组分别在含0.04、0.4、4、40 μmol/L UPA的培养液中孵育1h;另2组为空白对照组、二甲基亚砜(DMSO)对照组.检测各组精子活力、存活率、形态、DNA完整性、顶体反应、精子超活化、精子内游离钙离子浓度.结果:不同浓度的UPA体外处理精子,精子存活率、活力、形态与对照组(空白+DMSO)比较差异无统计学意义(P＞0.05).UPA浓度达到0.4 μmol/L时处理精子,精子损伤的比例和彗尾长度显著增加,精子超活化的比例降低(P＜0.01);顶体反应被抑制,精子内钙离子浓度也显著降低(P＜0.01).结论:UPA在体外可抑制精子的顶体反应和超活化,降低精子内钙离子浓度,可能与UPA致精子损伤有关.
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