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首页> 中文期刊> 《吉林大学学报（医学版）》 >T-2毒素对人肝癌HepG2细胞的增殖抑制作用及促凋亡作用

T-2毒素对人肝癌HepG2细胞的增殖抑制作用及促凋亡作用

         
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摘要

Objective: To investigate the inhibitory effect of T-2 toxin on proliferation of the human hepatocellular carcinoma HepG2 cells and its promotion effect of apoptosis.Methods:The HepG2 cells in the logarithmic phase were selected and divided into control group (without T-2 toxin)and experimental groups (given 0.25,2.50,25.00,250.00 and 2500.00 μg·L-1 T-2 toxin).After 24 h treatment,the morphology of cells was observed under inverted microscope;the inhibitory rate of proliferation of cells was determined by MTT assay;the cell cycle and apoptotic rate of cells were analyzed by flow cytometry;Hochest 33258 staining was used to observe the apoptotic morphology of cells;the activity of caspase-3 in HepG2 cells was detected.Results:After treated for 24 h,the inverted microscope observation results showed that the number of the cells in experimental groups was decreased significantly and the cells shrank and deformed.The MTT results showed that compared with control group,the inhibitory rates of proliferation of the cells in experimental groups were increased (P <0.01).The flow cytometry results showed that compared with control group, the percentage of the cells in SubG1 phase in experimental group was significantly increased,and the apoptotic rates of the cells in experimental groups were significantly increased.The Hoechest 33258 staining results showed that the chromatin condensation was observed in the cells in experimental groups,and the nuclei were dense and stained.Compared with control group,the activities of intracellular caspase-3 of the cells in experimental groups were significantly increased (P < 0.01 ). Conclusion:T-2 can inhibit the proliferation of human hepatocellular carcinoma HepG2 cells,and induce the apoptosis.%目的:探讨T-2毒素对人肝癌HepG2细胞增殖的抑制作用和促凋亡作用.方法:选取对数生长期的人肝癌HepG2细胞,分为对照组(未给予T-2毒素)和实验组(给予0.25、2.50、25.00、250.00和2500.00μg·L-1 T-2毒素).24 h后倒置显微镜下观察细胞形态变化,MTT法检测细胞增殖抑制率,流式细胞术检测细胞周期和细胞凋亡率,Hoechest 33258染色法观察HepG2细胞凋亡形态表现,检测各组HepG2细胞中caspase-3的活性.结果:T-2毒素作用HepG2细胞24 h后,倒置显微镜下观察,与对照组比较,实验组细胞数量明显减少,细胞皱缩变形.MTT法检测,与对照组比较,实验组细胞增殖抑制率升高(P<0.01).流式细胞术检测,与对照组比较,实验组SubG1期细胞比例明显升高,细胞凋亡率明显升高.Hoechest 33258染色法检测,实验组细胞出现染色质固缩,细胞核呈致密浓染色的凋亡形态.实验组细胞中caspase-3活性明显高于对照组(P<0.01).结论:T-2毒素对人肝癌HepG2细胞增殖具有明显的抑制作用,并能促进细胞凋亡.

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  • 来源
    《吉林大学学报（医学版）》 |2017年第6期|1087-1091前插2|共6页
  • 作者

    朱文赫; 柳玉; 张家琦; 李艳; 刘微; 董媛; 刘磊; 王会岩;

  • 作者单位

    吉林医药学院基础医学院生物化学教研室,吉林 吉林 132013;

    吉林医药学院检验学院生物技术教研室,吉林 吉林 132013;

    吉林医药学院检验学院生物技术教研室,吉林 吉林 132013;

    吉林医药学院检验学院生物技术教研室,吉林 吉林 132013;

    吉林医药学院检验学院生物技术教研室,吉林 吉林 132013;

    吉林医药学院检验学院生物技术教研室,吉林 吉林 132013;

    吉林医药学院检验学院生物技术教研室,吉林 吉林 132013;

    吉林医药学院检验学院生物技术教研室,吉林 吉林 132013;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 肝肿瘤;
  • 关键词

    T-2毒素; 肝肿瘤; 细胞凋亡; caspase-3;

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