目的探讨12C6+重离子射线对人肝癌HepG2细胞迁移、侵袭和黏附能力的影响及相关机制.方法选择对数生长期HepG2细胞,经0、1、2、4Gy剂量12C6+重离子射线辐照后,用Transwell法检测HepG2细胞迁移能力和侵袭能力,MTS法(内盐法)测定黏附能力,蛋白免疫印迹法检测基质金属蛋白-2(MMP-2)、MMP-9蛋白表达水平.结果12C6+重离子射线辐照后,与0Gy组相比,随着辐照剂量的增加,HepG2细胞迁移能力显著下降,侵袭能力也随之下降(P＜0.05);HepG2细胞黏附能力增强(P＜ 0.05);HepG2细胞中MMP-2和MMP-9表达量下调(P＜ 0.05).结论12C6+重离子辐照能增强HepG2细胞的黏附能力,抑制HepG2细胞的迁移能力和侵袭能力,这种作用可能与其下调MMP-2和MMP-9的表达有关.%Objective To investigate the effects of 12C6+ heavy ion irradiation on migration,invasion and adhesion of human hepatocellular carcinoma HepG2 cells and its related mechanisms.Methods The logarithmic HepG2 cells were irradiated by 0,1,2,4 Gy of 12C6+ heavy ion ray and then the migration and invasion were determined by Transwell method,the adhesion ability was detected by MTS method (inner salt method),and the matrix metalloproteinases-2 (MMP-2) and MMP-9 protein expression was detected by Western blot.Results After 12C6+ heavy ion rays irradiation,compared with the 0 Gy group,with the increase of radiation dose,HepG2 cell migration ability decreased significantly,the invasion ability also decreased (P ＜ 0.05);HepG2 cell adhesion ability enhanced (P ＜ 0.05);the amount of MMP-2 and MMP-9 expression in HepG2 cells were down-regulated (P ＜ 0.05).Conclusion 12C6+ heavy ion irradiation can enhance the adhesion ability and inhibit the migration and invasion ability of HepG2 cells,which may be related to the down-regulation of MMP-2 and MMP-9 expression.
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