首页> 中文期刊> 《医学理论与实践》 >郴州地区5岁以下住院儿童严重急性呼吸道感染病毒病原学研究基金项目:郴州市科技局资助重点项目(CZ2013065);郴州市第一人民医院重点项目(N2013-005).通讯作者:范楚平

郴州地区5岁以下住院儿童严重急性呼吸道感染病毒病原学研究基金项目:郴州市科技局资助重点项目(CZ2013065);郴州市第一人民医院重点项目(N2013-005).通讯作者:范楚平

         

摘要

目的:通过对郴州地区 2013-2014 年5岁以下住院儿童严重急性呼吸道感染病毒病原学的病例对照研究,了解该地区的病毒流行病学特征,为临床诊治和疾病预防控制提供参考.方法:收集2013年7月-2014年6月因严重急性呼吸道感染住院患儿的鼻咽拭子489份,同时采集年龄、时间相匹配的无感染症状对照244份,采用real-time RT-PCR方法检测呼吸道合胞病毒(RSV)、鼻病毒(HRV)、偏肺病毒(HMPV)、A型流感病毒(IFV-A)、B型流感病毒(IFV-B)、C型流感病毒(IFV-C)、副流感病毒1~4型(PIV1~4)、腺病毒(ADV)、博卡病毒(HBoV)、肠道病毒(EV)及冠状病毒(HCoV-OC43、HCoV-NL63、HCoV-229E、HCoV-HKU1).结果:489份病例组中有359份(73.41%)至少检测到1种病毒,RSV检出率最高,为 27.61%,其次为ADV 9.82%、HRV 9.41%、IFV-A 8.38%、PIV2 7.15%,HMPV 5.32%,HPeV未检出.244份对照中有65份(26.64%)至少检测到1种病毒,PIV2、IFV-B、HKU1、PIV4、HPeV未检出,检出最高的为HRV 10.25%,病例组病毒总检出率比对照组检出率高,且差异有统计学意义.除HRV 、冠状病毒、HBoV、IFV-C、PIV4、EV病例组与对照组检出率差异无统计学意义外,其他病毒病例组检出率比对照组明显高.各病毒主要检出年龄与季节都不同,病毒混合感染以RSV合并HRV感染为主.结论:RSV、ADV、IFV-A、IFV-B、HMPV、PIV1~3是郴州地区儿童SARTI的重要病原,以冬春季主要流行,12~35个月儿童易感,而HBoV、IFV-C、PIV4、EV、HRV和冠状病毒可能是重要的无症状潜伏感染病毒.%Objective:We conducted a case-control study on viral etiological of hospitalized children who are younger than 5 years with severe acute respiratory tract infection(SARTI) in Chenzhou from 2013 to 2014,to understand the virus epidemiological characteristics of this region,to provide reference for clinical diagnosis,disease prevention and control.Methods:489 hospitalized children (case patients) with SARTI and 244 randomly selected,concurrently sampled age-matched-asymptomatic individuals (control subjects) among children aged less than 5 years from July 2013 to June 2014 enrolled in this study.Nasopharyngeal and oropharyngeal swabs of participants were tested for respiratory syncytial virus(RSV),rhinovirus(HRV),human metapneumovirus(HMPV),human influenza virus A(IFV-A),human influenza virus B(IFV-B),human influenza virus C(IFV-C),Parainfluenza 1~4(PIV1~4),adenovirus(ADV),human bocavirus(HBoV),enterovirus(EV)and human coronavirus(HCoV-OC43,HCoV-NL63,HCoV-229E,HKU1) using real-time fluorescence polymerase chain reaction(RT-PCR).Results:Among the 489 case patients enrolled,at least one virus was detected in 359(73.41%),RSV was the most frequently detected virus(27.61%),followed by ADV(9.82%),HRV(9.41%),IFV-A(8.38%),PIV2(7.15%),HMPV(5.32%),and no HPeV was detected.Among the 244 control subjects enrolled,at least one viruse was detected in 65(26.64%) and no PIV2,IFV-B,HKU1,PIV4,HPeV were detected,the most commonly detected viruse were HRV(10.25%).The overall positive rate of viral detection of case patients was higher than control subjects.Besides HRV,HCoV,HBoV,IFV-C,PIV4 and EV,positive rate of other viral detection of case patients was higher than control subjects.The major age and season of different viral detection were differences.RSV and HRV co-infection were the most common during mixed infection.Conclusion:RSV,ADV,IFV-A,IFV-B,HMPV,PIV1~3 were important etiologies of children with SARTI in Chenzhou.The viral pathogens were detected mainly during winter and spring and were more often detected in 12 to 35 months patients.While HBoV,IFV-C,PIV4,EV,HRV and HCoV can be important asymptomatic latent infection viral pathogens.

著录项

  • 来源
    《医学理论与实践》 |2017年第7期|943-945,951|共4页
  • 作者单位

    南华大学附属郴州市第一人民医院 郴州市儿童哮喘防治技术研发中心,湖南省郴州市 423000;

    南华大学附属郴州市第一人民医院 郴州市儿童哮喘防治技术研发中心,湖南省郴州市 423000;

    南华大学附属郴州市第一人民医院 郴州市儿童哮喘防治技术研发中心,湖南省郴州市 423000;

    南华大学附属郴州市第一人民医院 郴州市儿童哮喘防治技术研发中心,湖南省郴州市 423000;

    南华大学附属郴州市第一人民医院 郴州市儿童哮喘防治技术研发中心,湖南省郴州市 423000;

    南华大学附属郴州市第一人民医院 郴州市儿童哮喘防治技术研发中心,湖南省郴州市 423000;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 小儿呼吸系及胸部疾病;
  • 关键词

    严重急性呼吸道感染; 住院儿童; 病毒病原学;

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