目的:探讨中药康莱特对人结肠癌裸鼠种植瘤肝转移的抑制作用.方法:将结肠癌LoVo细胞注入裸鼠脾脏,建立结肠癌肝转移模型.将成功制模的裸鼠随机分为4组:对照组,CTX组,康莱特组及CTX+康莱特组.治疗45天后,处死裸鼠,计算肝转移结节数.采用ELISA法测定肿瘤组织上清液骨桥蛋白(OPN)的浓度.结果:在肝转移结节数目上,与对照组相比其余3组肝转移结节个数均降低且有统计学意义.在组织骨桥蛋白浓度上,其余3组组织骨桥蛋白浓度降低且有统计学意义.与CTX组相比,CTX+康莱特组组织骨桥蛋白浓度降低且有统计学意义,但CTX组与康莱特组二者无统计学差异P=0.176.结论:康莱特可以抑制裸鼠结肠癌的肝转移,康莱特联合CTX具有协同作用.%Objective :To observe the effect of Kanglaite on liver metastasis in human colon carcinoma. Methods : LoVo cell were injected into nude mice. Twenty - four liver metastasis model nude mice were divided into four groups randomly : control group, CTX group , KLT group and CTX + KLT group. Animals were sacrificed after 45 days, and their livers were processed for histological examination. Tumor nodes in liver were counted and tissue osteopontin were dectected. Results: The 3 treated groups displayed a significant inhibitory effect on liver metastasis compared with the control not only in tumor nodes but also in tissue osteopontin. ( P ＜ 0. 05 ). Conclusion : KLT can inhibit the growth of liver metastasis tumors and KLT in combination with CTX can improve the effect.
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