Objective :To investigate the effects of Astragalus with active component of Chinese traditional medicine on the apoptosis of human lung adenocarcinoma cell line. Methods : Different concentrations of Astragalus were used in the culture of SPC - A - 1 cells. Proliferation and apoptosis of SPC - A - 1 cells were observed by MTT, immunocytochemistry, indirect immunofluorescene and flow cytometry quantitative analysis. The expression of Bcl - 2 and Bax protein was determined by qualitative analysis. Results : Proliferation of SPC - A - 1 cells was inhibited effectively after being treated by different concentrations of Astragalus. The rate of apoptosis was increasing with increased concentration of Astragalus. The expression of Bcl - 2 decreased in the experimental groups. Otherwise, the expression of Bax protein was significantly higher than the control group. Conclusion : Astragalus can inhibit proliferation and promote apoptosis of SPC - A - 1 cells effectively, the mechanism may be correlated to the up - regulation of Bax and down - regulation of Bcl - 2.%目的:探讨中药提取物黄芪(Astragalus)对人肺腺癌(lung adenocarcinoma)细胞系SPC-A-1的凋亡诱导作用及其发生机制.方法:体外培养SPC-A-1细胞,用不同浓度黄芪对体外培养的SPC-A-1细胞进行干预.分别采用MTT、免疫细胞化学染色、流式细胞仪法检测其对SPC-A-1细胞的增殖抑制和凋亡诱导作用;并对凋亡相关Bcl-2与Bax 蛋白表达的变化进行定性检测.结果:经不同浓度黄芪处理后 的SPC-A-1细胞,其生长受到明显的抑制,细胞凋亡指数随药物浓度增加而明显增加;黄芪作用Bax表达明显高于对照组.结论:一定浓度的黄芪能抑制SPC-A-1细胞的增殖,促进其凋亡;其机制可能与调控Bcl-2、Bax 表达有关.
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