目的 探讨参附对大鼠心肌缺血再灌注损伤的炎症反应的影响.方法 30只健康 SD大鼠随机分为假手术组(S组)、缺血再灌注组(I/R组)和参附组(SF组),每组10只.采用结扎左冠状动脉前降支的方法制备缺血再灌注模型.观察各实验组心肌组织病理形态变化,检测心肌组织匀浆中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量.结果 1)I/R组心肌组织结构紊乱,可见局灶性坏死,明显炎性细胞浸润;与 I/R组相比,SF组心肌损伤程度明显减轻,炎性病变显著减轻.2)与 I/R组相比,SF组大鼠心肌组织匀浆中 SOD活性升高,MDA水平降低.结论 参附能够降低缺血再灌注心肌炎性反应,从而减轻心肌组织缺血再灌注损伤.%Objective To investigate the effect of Shenfu injection on inflammatory response following myocardial ischemia-reperfusion injury in rats.Methods Thirty healthy SD rats were randomly divided into sham-operation group(S group),ischemia-reperfusion group(I/R group) and Shenfu injection group(SF group),with 10 rats in each group.The ischemia-reperfusion was induced by left anterior descending coronary artery ligation.Pathological changes,superoxide dis-mutase(SOD)activity and malondialdehyde(MDA)content in myocardial tissue were measured in all the three groups.Results Compared with S group,structural disorganization,focal necrosis and inflammatory cell infiltration were found in the myocardial tissue of I/R group.Compared with I/R group,myocardial injury,inflammatory lesion and MDA production were decreased and SOD activity was increased in SF group.Conclusion Shenfu injection can alleviate myocardial is-chemia-reperfusion injury through reducing inflammatory response.
展开▼
