Objective To investigate the effect of triptolide(TP)on urinary albumin excretion in rats with diabetic nephropathy(DN).Methods Sixty SD rats were randomly divided into nega-tive control group(n=8)and experimental group(n=52).Rats in experimental group were given streptozocin to establish the model of DN(four rats were excluded from the study because of inad-equate blood glucose levels).The 48 DN rats were randomly divided into four groups:DN group, 0.2 mg·kg-1 ·d-1 TP group(Tl group),0.4 mg·kg-1 ·d-1 TP group(Th group),and 10 mg· kg-1 ·d-1 benazepril group(Ben group),with 12 rats in each group.Intragastric administration was performed from week 5 after successful modeling once daily for 4 weeks.Detect blood glucose and 24-hour urinary in rats before and after the treatment.Results At model establishment, blood glucose levels increased in model rats compared with negative control group(P<0.01),but no significant differences were found among DN,Tl,Th and Ben groups(P>0.05).At 4 weeks after model establishment,compared with negative control group,24-hour urinary albumin excretion increased in DN,Tl,Th and Ben groups(P <0.01).At 8 weeks after model establishment, compared with DN group,24-hour urinary albumin excretion decreased in Tl,Th and Ben groups (P <0.01),and the decrease in Tl and Th groups was more obvious than that in Ben group(P < 0.05).Conclusion TP can effectively reduce urinary albumin excretion in DN rats.%目的 探讨中药雷公藤提取物雷公藤甲素(triptolide,TP)对糖尿病肾病(diabetic nephropathy,DN)大鼠尿微量白蛋白排泄的影响.方法 60只SD大鼠随机分为正常对照组(Control组,8只)和实验组(52只),实验组使用链脲佐菌素(streptozocin,STZ)建立DN模型(有4只大鼠血糖未达标,剔除实验).将48只造模成功的大鼠按照随机数字表法分为4组,每组12只:DN组、TP低剂量组(Tl组,予TP 0.2 mg·kg-1·d-1)、TP高剂量组(Th组,予TP 0.4 mg·kg-1·d-1)、贝那普利治疗阳性对照组(Ben组,予贝那普利10 mg·kg-1·d-1).成模后第5周开始灌胃治疗,每天1次,共治疗4周.治疗前后检测大鼠的血糖和24 h尿微量白蛋白.结果 DN组、Tl组、Th组、Ben组在糖尿病成模时血糖水平均较基础血糖明显升高(P<0.01),DN组、Tl组、Th组、Ben组各组间血糖水平比较差异均无统计学意义(均P>0.05).成模4周时DN组、Tl组、Th组和Ben组24 h尿微量白蛋白均较Control组增高(均P<0.01),提示DN已经发生;成模8周时Ben组、Tl组、Th组3组24 h尿微量白蛋白均较DN组显著降低(P<0.01),Tl组和Th组比Ben组降低更明显(P<0.05).结论 TP能显著减少DN大鼠尿微量白蛋白的排泄,是减少大鼠DN尿蛋白的有效药物.
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