Objective To investigate the distribution and drug resistance changes of blood cul-ture pathogens in Inner Mongolian People’s Hospital between 2009 and 2012.Methods Blood samples were cultured with the BacT/Alert 3D automated blood culture system.Bacterial identifi-cation and antibiotic susceptibility testing were performed using Vitek 2 system(bio-Mérieux, France).All data were analyzed using Whonet 5.6 software.Results A total of 659 strains of pathogens were isolated from blood samples between 2009 and 2012.The positive rate was 13.9%.Of the 659 strains,gram-negative bacilli and gram-positive cocci accounted for 54.5% and 42.9%,respectively.The detection rate was highest in Escherichia coli(25.6%),followed by co-agulase-negative staphylococcus(Staphylococcus aureus and staphylococcus epidermidis,23.9%). The positive blood culture pathogens were commonly found in department of urology(12.3%), ICU(12.5%) and department of general surgery (11.2%).Antibiotic susceptibility testing showed that Enterobacteriaceae strains were highly susceptible to carbapenem (100.0%),and were also sensitive to cefotetan,piperacillin/tazobactam and amikacin(>85.7%).No strains re-sistant to vancomycin were found in Staphylococcus and Enterococcus isolates.Linezolid and quinu-pristin/dalfopristin exhibited high activity against gram-positive cocci(>93.1%).Pseudomonas aerugi-nosa and Acinetobacter were highly resistant to multiple antimicrobial agents,except for amikacin,pip-eracillin/tazobactam,ampicillin/sulbactam,ciprofloxacin and levofloxacin(<17.1%).Conclusion The distribution and drug resistance changes of blood culture pathogens have their own characterris-tics,which can provide the basis for the treatment and prevention of diseases.%目的：了解内蒙古自治区人民医院2009-2012年血培养阳性标本的病原菌分布和耐药性变迁。方法采用 Bact/Alert 3D 全自动血培养仪，对血培养培养瓶进行连续培养监测，细菌鉴定和药敏试验采用法国生物梅里埃公司 VITEK 2-compact 全自动微生物分析系统，应用 Whonet5．6软件对数据进行统计分析。结果2009-2012年，该院共分离出659株病原菌，阳性率为13．9％。其中，革兰阴性杆菌和革兰阳性球菌所占比例分别为54．5％、42．9％。大肠埃希菌检出率最高，占25．6％，其次是凝固酶阴性葡萄球菌（人葡萄球菌和表皮葡萄球菌），占23．9％。血培养阳性菌多见于泌尿内科（占12．3％）、ICU（占12．5％）、普外科（占11．2％）。耐药性分析显示：血行感染肠杆菌科细菌对亚胺培南高度敏感（100．0％），其对头孢替坦、哌拉西林/他唑巴坦和阿米卡星也较敏感（＞85．7％）。未发现耐万古霉素的葡萄球菌和肠球菌，利奈唑胺和奎奴普丁/达福普丁也对革兰阳性球菌有较好的敏感性（＞93．1％）。铜绿假单胞菌和不动杆菌对多种抗菌药物的耐药率比较高，只有阿米卡星、哌拉西林/他唑巴坦、氨苄西林/舒巴坦、环丙沙星和左旋氧氟沙星耐药率较低（＜17．1％）。结论血培养病原菌分布和耐药性变迁有其特点，可为其治疗及预防提供依据。
展开▼
