Objective To evaluate the application value of immunohistochemistry combined with in situ hybridization for detecting immunoglobulin κ,λ light chain restriction in mantle cell lymphoma(MCL). Methods The expression of CyclinDl was detected by immunohistochemistry using Envision two step method in 15 cases of nodular mantle cell lymphoma (group 1) ,15 cases of diffuse mantle cell lymphoma group (group 2) and 10 cases of reactive lymph node hyperplasia (control group). The k to λ light chain ratio was measured by in situ hybridization to determine the light chain restriction of tumor cells. Results CyclinDl was positively expressed in nodular mantle cell lymphoma and diffuse mantle cell lymphoma (15/15,100. 0%) ,but was not expressed in the surrounding residues or lymphocytes with reactive hyperplasia. In group 1,κ light chain restriction was found in 7 cases (7/15,46. 7%), λ light chain restriction in 3 cases (3/15 ,20. 0%) , and no light chain restriction in 5 cases (5/15,33. 3%). In group 2,κ light chain restriction was found in 2 (2/15,13. 3%) ,A light chain restriction in 6 cases (6/15,40. 0%) , and no light chain rnrestriction in 7 cases (7/15,46. 7%). No cases showed light chain restriction in control group. Conclusion The light chain restriction and types can be detected by cyclin Dl immunohistochem-istry and in situ hybridization in different types of MCL. Therefore, the combined detection is important for early diagnosis of MCL.%目的 探讨免疫组织化学联合原位杂交检测套细胞淋巴瘤中免疫球蛋白κ、λ轻链限制性的应用价值.方法 运用免疫组织化学Envision二步法检测30例套细胞淋巴瘤[将30例套细胞淋巴瘤患者按不同的病理分期分为2组:1组(结节型套细胞淋巴瘤组,15例)和2组(弥漫型套细胞淋巴瘤组,15例)]和10例反应性增生淋巴结(对照组)组织中CyclinD1的表达,同时用原位杂交检测各组免疫球蛋白κ、λ轻链的比例,确定肿瘤细胞的轻链限制性.结果 1组、2组肿瘤细胞均CyclinD1阳性表达(15/15,100.0％,弱阳性～强阳性),周边残留或反应性增生的淋巴细胞不表达.1组中,7例呈κ轻链限制性(7/15,46.7％),3例呈λ轻链限制性(3/15,20.0％),5例无轻链限制性(5/15,33.3％).2组中,2例呈κ轻链限制性(2/15,13.3％),6例呈λ轻链限制性(6/15,40.0％),7例无轻链限制性(7/15,46.7％).对照组均无轻链限制性.结论 运用免疫组织化学检测CyclinD1与原位杂交检测轻链限制性相结合可以更准确地检测出不同型的套细胞淋巴瘤的轻链限制性及类型,可以作为诊断早期套细胞淋巴瘤的重要方法.
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