To study the effects of early exersice intervention on oxidative stress of dopaminergic neurons of a rat model of Parkinson's disease induced by 6-hydroxydopamine(6-OHDA).Male Sprague Dawley rats are randomly divided into four groups:Control group(n=12),Control+Ex group(n=12),PD group(n=20),PD+Ex group(n=20).Parkinson's rats are made by injecting 6-hydroxydopamine into medial forebrain bundle(MFB) in the right brain of rat.The trained groups are put on a treadmill to run for 30 min at the speed of 11 m·min-1,5 d·(7 d)-1 for four weeks.Injected 0.5 mg·kg-1 apomorphine to induce rotation after one week.Four weeks later,observed DJ-1 positive cells by PCR and western biotting and chromatometric method tested the activity of superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) as well as the contents of malondialdehyde(MDA) in substantia nigra.After operation 2 weeks and 4 weeks the number of abnormal rotation is decreased significantly in PD+Ex group than that in PD group(P<0.05).DJ-1 positive cells are significantly decrease in PD group more than those of the control group(P<0.01) and significantly increase in PD+Ex group than PD group(P<0.05).PD group the activities of GSH-Px and SOD are decreased and MDA is increased in substantia nigra with control group(P<0.01).Compared to PD group and PD+Ex group, early exersice intervention significantly elevated the activities of GSH-Px and SOD in substantia nigra ,while the MDA contents is reduced(P<0.05).Early exersice intervention increase the survival rate of DA neurons in substantia nigra of PD model rats,the mechanism is that exercise can improve the substantia nigra DA neurons DJ-1 related to oxidative stress resistance.%探讨早期运动干预对帕金森病(PD)模型大鼠黑质多巴胺(DA)能神经元的抗氧化保护机制.选清洁级SD大鼠64只分为4组:假手术组(Control,12只)、假手术运动组(Control+Ex,12只)、PD组(PD,20只)和PD运动组(PD+Ex,20只).单侧前脑内侧束(MFB)注射6-OHDA建立PD模型大鼠,运动组术后24 h开始进行持续4周跑台训练,11 m·min-1,30 min·d-1,5 d·(7 d)-1.采用阿扑吗啡(APO)诱导旋转检测行为变化,应用PCR和Western blotting检测黑质DJ-1变化,比色法检测黑质超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和丙二醛(MDA)指标.黑质DJ-1基因表达显示,PD+Ex组比PD组分别明显增加(P<0.05);黑质DJ-1蛋白表达显示, PD+Ex组较PD组有明显提升(P<0.05);PD组大鼠SOD、GSH-Px活力明显低于Control组(P<0.01),MDA含量显著高于Control组(P<0.01);PD+Ex组大鼠SOD、GSH-Px活力和MDA含量与Control组和PD组均有显著性差异(P<0.05).表明早期运动干预能提高黑质DA能神经元DJ-1并增强抗氧化应激能力.
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