目的:检测同一正畸力在不同时间微螺钉支抗周围液中白细胞介素-1β(IL-1β)和基质金属蛋白酶-9(MMP-9)的表达水平.方法:选取口腔正畸患者的39枚微螺钉,分别在种植钉植入后1周、4周和12周时收集其周围液.采用酶联免疫吸附法测定IL-1β和MMP-9的表达水平.结果:根据种植钉周围组织情况分为稳定组(n=27)和松动组(n=12),①松动组IL-1β在12周时浓度最高(P＜0.05).稳定组IL-1β在1周时表达最高,4周和12周之间的浓度无统计学差异.4周和12周时IL-1β在松动组表达水平较高(P＜0.05).②松动组MMP-9表达在12周时最高,稳定组中1周、4周和12周时MMP-9的表达无统计学差异.同一时间MMP-9表达水平在松动组表达较高(P＜0.05).结论:IL- 1β和MMP-9水平能够反映种植体微环境状况及微螺钉支抗稳定性.%Objective: To investigate interleukin-lβ( IL-lβ) and matrix metalloproteinases-9(MMP-9) levels in crevice fluid around microscrew anchorage. Methods; Crevice fluid around microscrew anchorage was collected from 39 microscrews of oral orthotic patients 1,4 and 12 weeks respectively after implantation, IL-lβ and MMP-9 were measured by enzyme linked immunosorbent assay. Results: The microscrews were divided into unstable group(n = 12) and stable group(n=27). In unstable group IL-lβ and MMP-9 expression levels increased from 1 to 12 weeks after implantation(P<0.05) and were higher than in stable group4 and 12 week after implantation(P<0.05). Conclusion; IL-1β and MMP-9 level may related to the stability of microscrew anchorage.
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