目的:研究不同fimA型牙龈卟啉单胞菌(Porphyromonas gingivalis,P.g)破坏上皮细胞间连接能力,探讨fimA基因型与致病力差异之间的关系.方法:应用不同fimA型P.g菌株ATCC 33277(Ⅰ型),WCSP115(Ⅱ型),WCSP1.5(Ⅲ型),W83 (Ⅳ型),3501(V型),4702(Ib型)分别与口腔上皮细胞系KB细胞在标准条件下共同孵育,于孵育后0.5、1和3h,收集细胞蛋白,应用Westen- blot检测N-钙粘素(N-cadherin)和β-连环素(β-Catenin)的动态表达.结果:与对照组比较,各fimA型P.g对KB细胞N-cadherin和β-Catenin蛋白的表达均有明显的降解作用；其中ⅡfimA型组N-cadherin和β-Catenin蛋白的表达量低于其它fimA型组.结论:P.g可破坏上皮细胞间连接成分,破坏上皮细胞的完整性,而Ⅱ型fimA型P.g水解上皮细胞间粘合连接蛋白的能力强于其它fimA型.提示fimA基因型的不同可能是P.g的致病力差异的基因基础.%Objective; To study the effects of Porphyromonas gingivalis{P. G) stains with different fimA genotypes on the proteolysis of cellular adherence junction components of epithelial KB cells. Methods: KB cells were challenged by P. G stains for different time periods and cell extracts were collected. Proteolysis of cellular adherence junction components, N-cadherin and β-Calenin were assayed by western-blots. Results; Degradation of N-cadherin and β-Catenin was detected after incubation of KB cells with different fimA genotypes oiP.g, while the stain with type Ⅱ fimA genotype showed the most potent effect. Conclusion: P. G may impair the integrity of epithelium. P. G with type Ⅱ genotype has the most efficient ability to degrade the adherence junction components of epithelial cells.
展开▼
