Objective:To observe the dynamic process of the development of dentin and enamel during their mineralization period. Methods: Dental germs in maxillae and mandibulae were obtained from spontaneously aborted 21 weeks and 32 weeks fetus. The samples were prepared and stained with modified Mallory's trichrome staining and observed under light microscope. Results: Different mineralized layers in different period of development appeared different color variation in the dental germs. Conciusion: Modified Mallory's trichrome staining method may be used to study the mineralization of dental germ.%目的:观察牙釉质和牙本质基质矿化过程的动态变化,为进一步阐明牙釉质和牙本质的形成机制提供依据。方法:21和32周自然流产的胎儿2例。分离含有牙胚的上、下颌骨, 固定脱钙,25 g/L火棉胶、石蜡双重包埋,常规组织学切片,分别用改良Mallory's三色和HE染色,镜下观察。结果:在牙胚矿化过程中,不同时期、不同矿化层呈现不同的颜色变化。结论:改良Mallory's三色法较HE染色更为清楚的观察到牙胚发育中组织矿化过程的变化。
展开▼