通过PCR技术扩增出人铁蛋白基因，经酶切后与表达载体质粒pGEX-4T-2连接，重组质粒转化感受态大肠杆菌，利用菌落PCR、质粒双酶切、测序，证实成功地构建了人铁蛋白基因表达载体，利用IPTG对重组菌进行诱导表达，通过尿素洗涤纯化目的蛋白用于制备抗体．%The ferritin gene was cloned from human genome using the PCR technology. After digested by the enzymes （BamH I, Sma I）, it was inserted into the plasmid pGEX-4T-2 to reconstruct the expression vector. Then the reconstructed plasmid was converted into E. coli whereby to confirm using the colony PCR, the enzyme digestion and the DNA sequencing. The recombinant protein was expressed after inducing by IPTG and then purified using urea for antibody preparation.
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