Objective The cloning , expression and bioinformatic analysis of TaLEA2 gene were carried out to lay the theoretical basis for salt-tolerance of wheat .[Method] The TaLEA2 gene was obtained by homology cloning .Its gene structures and protein characteristics were analyzed by bioinformatic soft-wares .Real-time PCR was used to analyze its expression .[Result and conclusion]The results showed that TaLEA2 contained a 981 bp ORF encoding 326 amino acids.Using bioinformatics analysis to predict and analyze TaLEA2, the protein had classic domains and characteristics of LEA 2, rich in Lys and lack of Cys, high hydrophilia (GRAVY, -0.405) and heat stability (Instability index, 25.28).The results of real-time PCR revealed that the expression of TaLEA2 had a constitutive expression , whose levels were different in different tissues .The expression was induced markedly by drought , salt, low-temperature and pathogenic bacteria in particular , but no change in treatment with ABA .Therefore , it is speculated that TaLEA2 can play a role not only in growth and development conditions but also in stress stage of wheat , especially in resistance to drought-stress.%【目的】克隆小麦Triticum aestivum晚期胚胎发育丰富蛋白基因并对其进行基因结构、蛋白特性及表达模式分析，为其耐盐性研究奠定理论基础．【方法】搜索小麦EST数据库，通过同源克隆分离并获得小麦晚期胚胎发育丰富蛋白基因，用生物信息学软件分析该基因结构及蛋白特性，荧光定量试验分析该基因的表达模式．【结果和结论】获得1条1100 bp的核苷酸序列，该序列包含了1个981 bp的开放阅读框，编码1个由326个氨基酸残基组成的蛋白，该蛋白含有2个LEA2结构域，属于第2组LEA蛋白，该蛋白命名为TaLEA2．生物信息学分析显示TaLEA2蛋白具有LEA2家族的典型特征，富含赖氨酸（Lys），不含半光氨酸（Cys），无明显的高级结构，平均亲水系数（GRA-VY）为-0.405，稳定系数为25.28，这种氨基酸组成及结构均有利于蛋白的热稳定性及亲水性．实时荧光定量PCR分析表明，TaLEA2基因为组成型表达，同时该基因存在组织表达差异，且表达受不同发育时期影响；该基因调控表达受高盐、低温、病原菌及干旱等胁迫的影响，尤其在干旱胁迫中上调表达明显，但该基因不受外源ABA刺激的影响．推测TaLEA2基因参与了小麦正常条件下的生长发育，同时也广泛地参与了小麦抵御逆境胁迫的响应，尤其在小麦的抗旱胁迫过程中发挥重要作用．
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